巨菌草对七彩山鸡生长、屠宰性能及H-FABP和A-FABP基因表达的影响  

Effects of Pennisetum giganteum on Growth,Slaughter Performance and Gene Expression of H-FABP and A-FABP in Phasianus colchicus

作　　者：郝柳柳 邓新为 裘博文 孙志宏[1] HAO Liuliu;DENG Xinwei;QIU Bowen;SUN Zhihong(College of Life Sciences,Yan’an University,Yan’an,Shaanxi 716000)

机构地区：[1]延安大学生命科学学院,陕西延安716000

出　　处：《中国农学通报》2022年第35期105-110,共6页Chinese Agricultural Science Bulletin

基　　金：2017年延安大学校级大学生创新创业训练计划项目“菌草配合饲料对禽类肉质品质的影响”(D2017086)。

摘　　要：本试验通过在基础饲粮中添加巨菌草,探究其对七彩山鸡生长指标、屠宰性能以及心脏型脂肪酸结合蛋白(H-FABP)和脂肪型脂肪酸结合蛋白(A-FABP)基因的影响。试验选取同批次、健康的七彩山鸡150只(4周龄),随机分为4个试验组和1个对照组,每组10只,3个重复,试验组巨菌草的添加量分别为4%、8%、12%、16%,对照组喂养基础饲粮,饲养周期为16周。结果发现,与对照组相比,添加8%的巨菌草组的平均体重差异显著提高(P<0.05);平均日采食量有所增加,但差异不显著(P>0.05),但各试验组的平均日增重相比较于对照组均显著提高(P<0.05),其中以8%的巨菌草组提高效果最为显著;添加量为4%、8%、12%的巨菌草组料重比有所下降;与对照组相比,8%巨菌草组的屠宰率、半净膛率和全净膛率显著升高(P<0.05);巨菌草组七彩山鸡胸肌率有所升高,但与对照组相比差异不显著(P>0.05);添加量为8%的巨菌草组的腿肌率较对照组显著提高(P<0.05)。经实时荧光定量PCR测定H-FABP和A-FABP基因在添加巨菌草饲粮之后均有差异表达,其中以8%巨菌草组显著提高。因此,饲粮中添加8%的巨菌草可显著改善七彩山鸡的生长指标、屠宰性能和脂肪相关基因的表达。The purpose of this experiment is to investigate the effect of adding Pennisetum giganteum in diet on growth indexes,slaughter performance,and fat-related genes of heart-type fatty acid-binding protein(HFABP)and adipocyte fatty acid-binding protein(A-FABP)gene in Phasianus colchicus.The experiment selected 150 healthy P.colchicus(4 weeks old)in the same batch and randomly divided them into 4 experimental groups and 1 control group,10 in each group,3 replicates.The addition amount for the experimental groups was 4%,8%,12%,and 16%,respectively.The control group was fed with basal diet,and the feeding period was 16 weeks.Compared with that of the control group,the difference of the average body weight of the 8%P.giganteum adding group was significantly increased(P<0.05);the average daily feed intake increased,but the difference was not significant(P>0.05);the average daily weight increase of each experiment group was significantly greater than that of the control group(P<0.05),among which the 8%P.giganteum adding group had the most significant improvement effect.The 4%,8%,and 12%P.giganteum adding groups had a decrease in the ratio of feed to weight;and compared with those of the control group,the slaughter rate,half evisceration rate and complete evisceration rate of the 8%P.giganteum adding group significantly increased(P<0.05).The breast muscle rate of P.colchicus in the P.giganteum adding groups increased,but the difference was not significant compared with that of the control group(P>0.05);the leg muscle rate in the 8%P.giganteum adding group was significantly higher than that in the control group(P<0.05).The differential expression of H-FABP and A-FABP genes were determined by real-time fluorescence quantitative PCR after the addition of P.giganteum diet,and were significantly increased in the 8%P.giganteum adding group.Therefore,adding 8%of P.giganteum to the diet can significantly improve the growth index,slaughter performance and the expression of fat-related genes in P.colchicus.

关 键 词：七彩山鸡 巨菌草 生长性能 屠宰性能 H-FABP基因 A-FABP基因 

分 类 号：S828.5[农业科学—畜牧学]