Application of CRISPR/Cas9 technology in forages  

作　　者：Qinyan Bao Tezera WWolabu Qian Zhang Tian Zhang Zhipeng Liu Juan Sun Zeng-Yu Wang 

机构地区：[1]College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou,China [2]College of Grassland Science,Qingdao Agricultural University,Qingdao,China [3]Institute for Agricultural Biosciences,Oklahoma State University,Ardmore,Oklahoma,USA

出　　处：《Grassland Research》2022年第4期244-251,共8页草地研究（英文）

基　　金：National Natural Science Foundation of China(U1906201).

摘　　要：Genome editing is an advanced genetic modification tool that facilitates the removal,insertion,or substitution of nucleotides in the genome of an organism for mutation induction.CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)is the most widely used genome editing tool that can precisely modify the specific sequence of a genome.Significant progress has been made in the use of CRISPR/Cas9 for the genetic improvement of food crops.However,in forage crops,the use of CRISPR/Cas9 is still in the infant stage with limited understanding and application.This article reviews current advances in the establishment and application of the CRISPR/Cas9 system in alfalfa,summarizes the application of CRISPR/Cas9 in several other forage legume and grass species,and discusses the prospects of CRISPR/Cas9 in the genetic improvement of forage crops.

关 键 词：ALFALFA CRISPR/Cas9 forage grass forage improvement forage legume genome editing 

分 类 号：S812[农业科学—草业科学]