3种何首乌单体成分致SD大鼠急性肾脏损伤组织病理学研究  

Histopathology of acute kidney injury in SD rats induced by three monomer components of Polygoni Multiflori Radix

作　　者：霍桂桃[1] 文海若 杨艳伟[1] 秦超 汪祺[1] 马双成[1] HUO Guitao;WEN Hairuo;YANG Yanwei;QIN Chao;WANG Qi;MA Shuangcheng(National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《中国药物警戒》2022年第12期1303-1308,共6页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(81503347)。

摘　　要：目的研究连续14 d经口灌胃给予SD大鼠3种何首乌单体成分体内急性肾毒性形态学特征。方法50只雄性SD大鼠随机分为对照组,大黄素-8-O-β-D-葡萄糖苷28、280、1120 mg·kg^(-1)剂量组,大黄素型单蒽酮6.5、65、650 mg·kg^(-1)剂量组及大黄素甲醚6.5、65、650 mg·kg^(-1)剂量组。连续14 d经口灌胃给予,给药结束后进行动物麻醉及剖检,并对主要脏器进行大体病理学检查及称重,而后对固定的组织脏器进行制片和组织病理学检查。结果大体病理学检查仅大黄素型单蒽酮650 mg·kg^(-1)剂量组1只大鼠双侧肾脏可见白色结节,对照组,大黄素-8-O-β-D-葡萄糖苷3个剂量组,大黄素型单蒽酮6.5、65 mg·kg^(-1)剂量组以及大黄素甲醚3个剂量组的大鼠均未见大体病理学改变。与对照组相比,3种何首乌单体成分各剂量组动物肾脏的绝对质量及脏/体比均未见统计学差异(P>0.05)。组织病理学检查发现大黄素-8-O-β-D-葡萄糖苷可引起肾脏肾小管透明小滴生成;大黄素型单蒽酮可引起肾脏肾小管色素沉着,并且加重肾小管嗜碱性变;大黄素甲醚未对肾脏造成明显的急性损伤。结论连续14 d经口灌胃给药,大黄素-8-O-β-D-葡萄糖苷和大黄素型单蒽酮可引起SD大鼠肾脏发生一定的形态学改变,而大黄素甲醚未对SD大鼠肾脏造成形态学改变。Objective To study the morphology of acute renal injury caused by three Polygoni Multiflori Radix monomers in SD rats after 14 days of oral administration.Methods Fifty male SD rats were randomly divided into the control group,the 28 mg·kg^(-1),280 mg·kg^(-1)and 1120 mg·kg^(-1)dose of emodin-8-O-β-D-glucoside groups,the 6.5 mg·kg^(-1),65 mg·kg^(-1)and 650 mg·kg^(-1)dose groups of emodin-type monoanthranone and the 6.5 mg·kg^(-1),65 mg·kg^(-1)and 650 mg·kg^(-1)dose groups of physcion.All doses of emodin-8-O-β-D-glucoside,emodin-type monoanthranone and physcion were administered orally for 14 days,respectively.After administration,the animals were anesthetized and necropsied,the kidneys were examined for macroscopic observation and weighed before the fixed kidneys were subjected to section preparation and histopathological examination.Results Macroscopic observation showed white nodules in bilateral kidneys of one rat in the 650 mg·kg^(-1)dose group of emodin-type monoanthranone.There were no gross lesions in SD rats in the three dose groups of emodin-8-O-β-D-glucoside,the 6.5 mg·kg^(-1),65 mg·kg^(-1)dose groups of emodin-type monoanthranone,or in the three dose groups of physcion.Compared with the control group,there were no statistically significant differences in the absolute kidney weight or organ/body weight ratio of emodin-8-O-β-D-glucoside,emodin-type monoanthranone and physcion in each dose group(P>0.05).Microscopic observation showed that emodin-8-O-β-D-glucoside could ca use the formation of tubular hyaline droplets,emodin-type monoanthranone could induce tubular pigmentation and aggravate tubular basophilia,and that physcion did not cause obvious injury to the kidney.Conclusion Emodin-8-O-β-D-glucoside and emodin-type monoanthranone can lead to morphological changes in the kidney of SD rats,but physcion does not.

关 键 词：何首乌 大黄素-8-O-β-D-葡萄糖苷 大黄素型单蒽酮 大黄素甲醚 大鼠 肾毒性 形态学特征 

分 类 号：R994.1[医药卫生—毒理学]