吴茱萸不同提取方式差异化学物质和毒性物质基础研究  

Variations of chemical substances and toxic substances of Evodia rutaecarpa using different extraction methods

作　　者：姬海南 佟娜 刘畅 赵潺 周晓娟 隋峰[5] 沈国林[1,2] JI Hainan;TONG Na;LIU Chang;ZHAO Chan;ZHOU Xiaojuan;SUI Feng;SHEN Guolin(Institute of Chemicals Safety,Chinese Academy of Inspection and Quarantine,Beijing 100123,China;Key Laboratory of Food Quality and Safety for State Market Regulation,Beijing 100123,China;Center for Food Evaluation,State Administration for Market Regulation,Beijing 100022,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100094,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100170,China)

机构地区：[1]中国检验检疫科学研究院化学品安全研究所,北京100123 [2]国家市场监督管理总局食品质量安全重点实验室,北京100123 [3]国家市场监督管理总局食品审评中心,北京100022 [4]中国医学科学院北京协和医学院药用植物研究所,北京100094 [5]中国中医科学院中药研究所,北京100170

出　　处：《中国药物警戒》2022年第12期1309-1314,1320,共7页Chinese Journal of Pharmacovigilance

基　　金：国家市场监督管理总局技术保障专项(2022YJ28);国家重点研发计划(2017YFF0211201);国家市场监督管理总局化学品质量安全检测能力提升专项(15002000000019)。

摘　　要：目的研究吴茱萸不同提取方式存在差异性的化学物质以及引起肝脏毒性可能的主要物质基础。方法分别制备吴茱萸水提组分、30%乙醇提组分、50%乙醇提组分和80%乙醇提组分,采用植物组学方法找出差异性化学物质,利用软件预测方法筛查主要差异性化学物质的毒性终点。结果吴茱萸水提与不同浓度醇提相比较获得的差异性化学物质总共有50种,其中最主要的差异性化学物质是吴茱萸生物碱,毒性终点筛查的结果显示吴茱萸生物碱存在安全风险,并揭示了吴茱萸醇提取物的毒性大于水提取物的原因。结论不同提取方式可以使吴茱萸存在差异性化学物质以及吴茱萸生物碱可能是其毒性物质基础。Objective To study the chemical substances of Evodia rutaecarpa extracted using different methods and the possible main material basis of hepatotoxicity.Methods The water extract,30% ethanol extract,50% ethanol extract and 80% ethanol extract of Evodia rutaecarpa were prepared respectively.The differential chemical substances were identified using the method of phytomics,and the toxicity of the main differential chemical substances was screened via software prediction.Results The results showed that there were 50 types of different chemical substances in water extraction and ethanol extraction of Evodia rutaecarpa,among which the most important one was Evodia alkaloids.The results of toxicity endpoint screening showed that there were safety risks in Evodia rutaecarpa alkaloids.The toxicity of Evodia rutaecarpa alcohol extract was higher than that of water extract.Conclusion Different extraction methods can make a difference to chemical substances of Evodia rutaecarpa,and Evodia alkaloids may be the basis of its toxic substances.

关 键 词：吴茱萸 植物组学 毒性物质基础 软件预测 

分 类 号：R927[医药卫生—药学]