丹参多酚酸盐对大鼠离体心脏心肌缺血再灌注损伤的保护作用及其机制  

Protective Effect and Mechanism of Salvianolate on Myocardial Ischemia-Reperfusion Injury in Isolated Rat Heart

作　　者：王永贤 贾敏 高景峰[3] 霍涛[4] 许立刚[4] WANG Yongxian;JIA Min;GAO Jingfeng;HUO Tao;XU Ligang(Catheterization Room,Shijiazhuang People's Hospital,Shijiazhuang 050000,China;Department of Cardiology,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;CT Room,Tangshan Fengrun People's Hospital,Tangshan 063000,China;Department of Cardiology,Baoding Third Central Hospital,Baoding 071000,China)

机构地区：[1]河北省石家庄市人民医院导管室,050000 [2]河北医科大学第二医院心内科,河北省石家庄市050000 [3]河北省唐山市丰润区人民医院CT室,063000 [4]河北省保定市第三中心医院心内科,071000

出　　处：《实用心脑肺血管病杂志》2023年第2期74-79,共6页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

基　　金：河北省卫生健康委医学科学研究课题(20210865)。

摘　　要：目的探讨丹参多酚酸盐对大鼠离体心脏心肌缺血再灌注损伤的保护作用及其机制。方法本实验时间为2021年2—8月。选取50只SD雄性大鼠,采用简单随机抽样法分为假手术组、模型组、丹参多酚酸盐组、己糖激酶Ⅱ(HKⅡ)抑制剂组、HKⅡ抑制剂+丹参多酚酸盐组,每组10只。模型组、丹参多酚酸盐组、HKⅡ抑制剂组、HKⅡ抑制剂+丹参多酚酸盐组通过Langendorff离体心脏灌流技术建立离体心脏心肌缺血再灌注损伤模型,其中假手术组不关闭灌流液;模型组建模后给予含有0.9%氯化钠溶液(20 mg/kg)的灌流液灌流;丹参多酚酸盐组建模后给予含有丹参多酚酸盐(20 mg/kg)的灌流液灌流;HKⅡ抑制剂组建模后给予含有HKⅡ抑制剂(10 mg/kg)的灌流液灌流;HKⅡ抑制剂+丹参多酚酸盐组建模后给予含有丹参多酚酸盐(20 mg/kg)及HKⅡ抑制剂(10 mg/kg)的灌流液灌流。检测五组大鼠心脏组织细胞色素C含量、腺苷酸〔三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、单磷酸腺苷(AMP)〕含量,线粒体通透性转换孔(mPTP)吸光度及线粒体中HKⅡ、Bax表达水平。结果模型组心脏组织细胞色素C含量高于假手术组,丹参多酚酸盐组低于模型组,HKⅡ抑制剂组高于模型组,HKⅡ抑制剂+丹参多酚酸盐组高于丹参多酚酸盐组(P<0.05)。模型组心脏组织ATP、ADP含量低于假手术组,丹参多酚酸盐组心脏组织ATP、ADP含量高于模型组,HKⅡ抑制剂组心脏组织ATP含量低于模型组,HKⅡ抑制剂+丹参多酚酸盐组心脏组织ATP、ADP含量低于丹参多酚酸盐组(P<0.05)。模型组mPTP吸光度高于假手术组,丹参多酚酸盐组低于模型组,HKⅡ抑制剂组高于模型组,HKⅡ抑制剂+丹参多酚酸盐组高于丹参多酚酸盐组(P<0.05)。模型组线粒体中HKⅡ表达水平低于假手术组、Bax表达水平高于假手术组,丹参多酚酸盐组线粒体中HKⅡ表达水平高于模型组、Bax表达水平低于模型组,HKⅡ抑制剂Objective To investigate the protective effect and mechanism of salvianolate on myocardial ischemiareperfusion injury in isolated rat heart.Methods This experiment lasted from February to August 2021.Fifty SD male rats were randomly divided into sham operation group,model group,salvianolate group,hexokinaseⅡ(HKⅡ)inhibitor group,HKⅡinhibitor+salvianolate group,with 10 rats in each group.The model group,salvianolate group,HKⅡinhibitor group,HKⅡinhibitor+salvianolate group were used to establish myocardial ischemia-reperfusion injury model by Langendorff isolated heart perfusion technique.In the sham operation group,the perfusion fluid was not closed;the model group was given perfusion solution containing 0.9%sodium chloride solution(20 mg/kg)after modeling;the salvianolate group was given perfusion solution containing salvianolate(20 mg/kg)after modeling;the HKⅡinhibitor group was given perfusion solution containing HKⅡinhibitor(10 mg/kg)after modeling;the HKⅡinhibitor+salvianolate group was given perfusion solution containing salvianolate(20 mg/kg)and HKⅡinhibitor(10 mg/kg)after modeling.The cytochrome C content and adenylate[adenosine triphosphate(ATP),adenosine diphosphate(ADP),adenosine monophosphate(AMP)]content in heart tissues,mitochondrial permeability transition pore(mPTP)absorbance,HKⅡand Bax expression levels in mitochondria of the five groups were detected.Results The cytochrome C content in heart tissues of the model group was higher than that of the sham operation group,cytochrome C content in heart tissues of the salvianolate group was lower than that of the model group,cytochrome C content in heart tissues of the HKⅡinhibitor group was higher than that of the model group,cytochrome C content in heart tissues of the HKⅡinhibitor+salvianolate group was higher than that of the pretreatment group(P<0.05).The ATP and ADP contents in heart tissues of the model group were lower than those of the sham operation group,ATP and ADP contents in heart tissues of the salvianolate group wer

关 键 词：心肌再灌注损伤 丹参多酚酸盐 己糖激酶Ⅱ 线粒体 

分 类 号：R542.2[医药卫生—心血管疾病]