高甘油三酯对人主动脉平滑肌细胞凋亡和炎症因子的影响  

Effects of high triglyceride on apoptosis and inflammatory factors of human aortic smooth muscle cells

作　　者：蒲丽君[1] 刘杰[1] 胡厚祥[1] PU Li-jun;LIU Jie;HU Hou-xiang(Cardiovascular Research Center,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院附属医院心血管疾病研究室,四川南充637000

出　　处：《川北医学院学报》2023年第1期6-9,共4页Journal of North Sichuan Medical College

基　　金：四川省南充市市校科技战略合作项目(18SXHZ0349)。

摘　　要：目的:探讨高甘油三酯(HTG)对人主动脉平滑肌细胞(HASMC)凋亡和炎症因子白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的影响。方法:不同浓度的甘油三酯(TG)干预HASMC 24 h,采用四甲基偶氮唑蓝(MTT)法检测细胞抑制率;流式细胞仪检测细胞凋亡;Western blot检测B细胞淋巴瘤/白血病-2(Bcl-2)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)蛋白的表达水平;酶联免疫吸附试验(ELISA)检测IL-6和TNF-α的水平。结果:与对照组相比,TG 2.5 mmol/L和5 mmol/L组凋亡率增加(P<0.05),且TG 5 mmol/L组凋亡率高于2.5 mmol/L组(P<0.05),而TG 1 mmol/L组凋亡率差异无统计学意义(P>0.05);TG处理细胞后,2.5 mmol/L和5 mmol/L组中Bcl-2蛋白表达降低(P<0.05),Caspase-3蛋白表达均升高(P<0.05),2.5 mmol/L和5 mmol/L组中IL-6和TNF-α浓度均升高(P<0.05),呈浓度依赖性。结论:中高浓度TG可促进HASMC凋亡,促使炎症因子IL-6和TNF-α的产生增加。Objective:To observe the effects of high triglyceride(HTG) on apoptosis of human aortic smooth muscle cells(HASMC) and inflammatory factors interleukin-6(IL-6) and tumor necrosis factor α(TNF-α).Methods:HASMC was treated with different concentrations of triglyceride(TG) for 24h.The cell inhibition rate was detected by MTT assay, cell apoptosis was detected by flow cytometry, protein expression of B-cell lymphoma-2(Bcl-2) and Caspase-3 was detected by Western blot, and the levels of IL-6 and TNF-α were detected by enzyme-linked immunosorbent assay.Results:Compared with the control group, the apoptosis rate of TG 2.5 mmol/L and 5 mmol/L groups was increased(P<0.05),and the apoptosis rate of TG 5 mmol/L group was higher than that of 2.5 mmol/L group(P<0.05),there was no statistical difference in the apoptosis rate of TG 1 mmol/L group(P>0.05).After TG treatment, Bcl-2 protein expression was decreased in 2.5 mmol/L and 5 mmol/L groups(P<0.05),Caspase-3 protein expression was increased(P<0.05),the concentrations of IL-6 and TNF-α in 2.5 mmol/L and 5 mmol/L groups were increased(P<0.05) in a concentration-dependent manner.Conclusion:Medium and high concentrations TG can promote the apoptosis of HASMC and increase the production of inflammatory factors IL-6 and TNF-α.

关 键 词：动脉粥样硬化 高甘油三酯 主动脉平滑肌细胞 凋亡 炎症因子 BCL-2 CASPASE-3 

分 类 号：R541.4[医药卫生—心血管疾病]