亚麻荠FUS3转录因子的鉴定及功能分析  

Identification and Functional Analysis of FUS3 Transcription Factor in Camelina sativa(L.) Crantz

作　　者：罗涛 王计平[1] 高慧玲 崔红利 李润植[1] LUO Tao;WANG Jiping;GAO Huiling;CUI Hongli;LI Runzhi(Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University,Taigu,Shanxi 030801,China)

机构地区：[1]山西农业大学、分子农业与生物能源研究所,山西太谷030801

出　　处：《西北植物学报》2022年第12期2003-2012,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：山西省基础研究计划(自由探索类)(20210302123418);山西省研究生创新项目(2021Y336);山西农业大学农学院育种工程专项重点培育项目(YZ2021-08)。

摘　　要：FUS3转录因子是调控植物种子油脂合成的关键因子。为探讨亚麻荠CsFUS 3基因在脂质合成和积累过程中的作用,该研究对CsFUS 3基因家族进行全基因组鉴定,分析CsFUS 3基因的时空表达模式,并解析CsFUS 3-1和CsFUS 3-2基因在植物油脂合成中的功能,为深入解析CsFUS 3基因在亚麻荠油脂合成中的功能及亚麻荠高油品种选育提供理论基础。结果表明:(1)利用AtFUS3蛋白序列,在亚麻荠基因组数据库中鉴定出2条完整的CsFUS3蛋白序列,分别命名为CsFUS3-1和CsFUS3-2,亚细胞定位发现2个CsFUS3蛋白均位于细胞核。(2)亚麻荠CsFUS3-1和CsFUS3-2蛋白与拟南芥AtFUS3蛋白的亲缘关系最近,具有与拟南芥AtFUS3蛋白相似的理化性质、高级结构以及完整的B3功能域。(3)qRT-PCR结果显示,CsFUS 3-1和CsFUS 3-2基因仅在种子中表达,且随着种子的发育成熟,2个CsFUS 3基因的表达量均呈先增高后降低的变化趋势,并在花后30 d时表达量达到最高。(4)CsFUS3和CsWRI1蛋白互作以及CsFUS 3对OLE和ABI 3基因的转录调控可能是亚麻荠高油性状的关键调控途径。(5)烟草瞬时表达分析表明,与野生型相比,转CsFUS 3-1和CsFUS 3-2基因的烟草叶片总油脂含量分别提高了0.95%和1.12%,表明亚麻荠CsFUS 3基因能够提高烟叶总油脂的合成积累。FUS3 transcription factor is a key regulator of seed oil synthesis in plants.In order to investigate the role of the CsFUS 3 gene in lipid synthesis and accumulation of Camelina sativa,we conducted a genome-wide identification of the CsFUS 3 gene family,analyzed the spatio-temporal expression patterns of CsFUS 3 genes,and analyzed the function of CsFUS 3-1 and CsFUS 3-2 genes in lipid synthesis.This study provides a theoretical foundation for in-depth analysis of the function of CsFUS 3 genes in the oil synthesis and the breeding of high oil varieties of C.sativa.The results showed:(1)using the AtFUS3 protein sequence,two complete CsFUS3 protein sequences were identified from the C.sativa genome database,named CsFUS3-1 and CsFUS3-2,respectively.The subcellular localization result showed that the two CsFUS3 proteins were located in the nucleus.(2)CsFUS3-1 and CsFUS3-2 proteins of C.sativa were most closely related to Arabidopsis AtFUS3 protein.They had similar physicochemical properties,advanced structure,and complete B3 functional domain to Arabidopsis AtFUS3 protein.(3)The results of qRT-PCR showed that the CsFUS 3-1 and CsFUS 3-2 genes were only expressed in seeds,and with the seeds matured,the expression levels of the two CsFUS 3 genes showed a trend of increasing at first and then decreasing,and the expression level reached the highest level at 30 days after flowering.(4)The interaction of CsFUS3 and CsWRI1 proteins and the transcriptional regulation of OLE and ABI 3 genes by CsFUS 3 may be key regulatory pathways of high oil traits in C.sativa.(5)The transient expression analysis of tobacco showed that compared with the wild type,the total oil content of tobacco leaves transfected with CsFUS 3-1 and CsFUS 3-2 genes increased by 0.95%and 1.12%,respectively.It indicated that the CsFUS 3 genes could increase the synthesis and accumulation of total oil in tobacco leaves.

关 键 词：亚麻荠 FUS3转录因子 表达模式 油脂代谢 

分 类 号：Q786[生物学—分子生物学] Q789