适配子修饰去细胞瓣支架促进再内皮化  

Modifying Decellularized Scaffolds with Aptamer for Re-endothelialization

作　　者：陈雪[1] 董念国[1] 史嘉玮[1] 徐旭[1] 周颖[1] 乔韡华 洪昊[1] Chen Xue;Dong Nianguo;Shi Jiawei(Department of Cardiovascular Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)

机构地区：[1]华中科技大学同济医学院附属协和医院心脏大血管外科,武汉430022

出　　处：《华中科技大学学报（医学版）》2022年第6期813-818,共6页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.81873502)。

摘　　要：目的研究将适配子固定在去细胞瓣支架表面加速瓣膜再内皮化的作用。方法制备3组主动脉瓣膜支架:去细胞瓣组、适配子固定的去细胞瓣组(适配子组)、戊二醛交联的去细胞瓣组(戊二醛组)。用流式细胞仪检测适配子与内皮祖细胞的结合效率;荧光显微镜检测适配子在去细胞瓣表面的固定;通过混合培养粘附实验检测内皮祖细胞在支架上的粘附和增殖能力。结果适配子与内皮祖细胞的结合实验显示适配子对内皮祖细胞具有特异性;荧光显微镜显示适配子被固定于去细胞瓣支架上;细胞培养实验显示适配子组支架上粘附的内皮祖细胞数量高于其余两组支架并且增殖较快。结论适配子能够促进内皮祖细胞在去细胞瓣支架上的特异性粘附和增殖,从而促进再内皮化。Objective To immobilize the aptamer onto decellularized porcine aortic heart valves for accelerating endothelialization.Methods In this study,three groups of scaffolds were constructed:decellularized porcine aortic heart valves(DAVs),aptamer-immobilized DAVs(aptamer-DAVs),and glutaraldehyde crosslinked DAVs(GA-DAVs)groups.The binding efficiency between the aptamer and EPCs was verified by flow cytometry and the immobilization of the aptamer onto DAVs was also detected.Then the adhesion of EPCs on scaffolds was tested via co-culture.Cells proliferation assay was used to evaluate the proliferation of EPCs on scaffolds.Results The results revealed that the binding between the aptamer and EPCs was specific,and the aptamer was immobilized onto DAVs.Cells adhesion experiments demonstrated that the number of adherent EPCs on aptamer-DAVs group was higher than other two groups of scaffolds.And EPCs seeded on aptamer-DAVs group grew faster than DAVs group and GA-DAVs group.Conclusion Aptamer could specifically promote the adhesion and proliferation of EPCs on DAVs,and promote re-endothelialization of scaffolds.

关 键 词：适配子 去细胞瓣 再内皮化 

分 类 号：Q819[生物学—生物工程] R654.2[医药卫生—外科学]