血清二胺氧化酶和肠脂肪酸结合蛋白测定评价窒息新生儿胃肠功能障碍的临床研究  

Clinical trial of serum diamine oxidase and intestinal fatty acid-binding protein in evaluating gastrointestinal dysfunction in neonates with asphyxia

作　　者：杨敏[1] 程雁[1] YANG Min;CHENG Yan(Department of Pediatrics,The Second Affiliated Hospital of Anhui Medical University,Hefei 230000,Anhui Province,China)

机构地区：[1]安徽医科大学第二附属医院儿科,安徽合肥230000

出　　处：《中国临床药理学杂志》2022年第23期2803-2806,共4页The Chinese Journal of Clinical Pharmacology

基　　金：安徽省卫生和计划生育委员会科研计划基金资助项目(2018SEYL019);安徽省医学会急诊临床研究基金资助项目(KY2018013)。

摘　　要：目的研究血清二胺氧化酶(DAO)和肠脂肪酸结合蛋白(I-FABP)测定对窒息新生儿胃肠功能障碍临床意义。方法入选本院收治的38例窒息新生儿作为试验组,将同期30例无窒息、感染、消化系统疾病的健康新生儿作为对照组。所有窒息新生儿接受标准的复苏方案,健康新生儿不采取额外的干预措施。用酶联免疫吸附法检测新生儿外周血血清DAO和I-FABP水平。分别比较试验组和对照组新生儿,试验组内轻度窒息和重度窒息的新生儿,试验组内肠道轻度损伤和重度损伤的新生儿外周血DAO、I-FABP水平差异,并分析新生儿外周血DAO、I-FABP水平与Apgar评分和急性肠道损伤(AGI)分级的相关性。结果对照组和试验组新生儿的出生第1天DAO水平分别为(5.87±1.23)和(17.89±3.50)U·L^(-1),I-FABP水平分别为(48.33±6.12)和(220.60±39.57)ng·L^(-1);出生第5天DAO水平分别为(5.83±1.02)和(8.93±2.51)U·L^(-1),I-FABP水平分别为(41.24±6.59)和(110.79±27.35)ng·L^(-1)。与对照组相比,试验组新生儿第1天和第5天DAO、I-FABP水平差异均有统计学意义(均P<0.05)。试验组轻度窒息和重度窒息新生儿的第1天DAO水平分别为(14.96±2.12)和(20.53±2.10)U·L^(-1);I-FABP水平分别为(184.60±21.97)和(253.00±16.86)ng·L^(-1)。与轻度窒息新生儿相比,重度窒息的新生儿上述指标差异均有统计学意义(均P<0.05)。试验组肠道轻度损伤新生儿和重度损伤新生儿第1天DAO水平分别为(14.59±1.78)和(20.29±2.24)U·L^(-1);I-FABP水平分别为(184.71±18.39)和(246.70±28.67)ng·L^(-1)。与肠道轻度损伤的新生儿相比,肠道严重损伤的新生儿上述指标差异均有统计学意义(均P<0.05)。Spearman秩相关分析表明,新生儿外周血DAO和I-FABP水平与Apgar评分和AGI分级均相关。结论血清DAOT和I-FABP水平与胃肠功能障碍具有良好相关性,可作为早期诊断新生儿窒息性胃肠功能障碍的指标。Objective To study the clinical significance of serum diamine oxidase(DAO)and intestinal fatty acid binding protein(I-FABP)on gastrointestinal dysfunction in asphyxiated neonates.Methods 38neonates with asphyxia admitted to our hospital were selected as the treatment group,and 30 healthy neonates without asphyxia,infection and digestive system diseases during the same period were selected as the control group.All asphyxiated neonates received a standard resuscitation plan and healthy neonates.No additional interventions were taken.The levels of serum DAO and I-FABP in the peripheral blood of neonates were detected by enzyme-linked immunosorbent assay.To compare the differences in peripheral blood DAO and I-FABP levels between treatment and control neonates,mild and severe asphyxia,and mild and toxic intestinal injury within the treatment group,and to analyze the correlation between peripheral blood DAO and I-FABP levels and Apgar score and AGI classification in neonates.Results The DAO levels of the neonates in the control group and the treatment group on the first day of life were(5.87±1.23)and(17.89±3.50)U·L^(-1),and the I-FABP levels were(48.33±6.12)and(220.60±39.57)ng·L^(-1),respectively;DAO levels on the fifth day of birth were(5.83±1.02)and(8.93±2.51)U·L^(-1),and I-FABP levels were(41.24±6.59)and(110.79±27.35)ng·L^(-1),respectively.Compared with the control group,the DAO and I-FABP levels of neonates in the treatment group on the 1st and 5th day were significantly different(all P<0.05).The DAO levels on day 1 of neonates with mild asphyxia and severe asphyxia in the treatment group were(14.96±2.12)and(20.53±2.10)U·L^(-1);the levels of I-FABP were(184.60±21.97)and(253.00±16.86)ng·L^(-1),respectively.Compared with the neonates with mild asphyxia,the differences of the above two indicators in the neonates with severe asphyxia were statistically significant(all P<0.05).The DAO levels of the neonates with mild intestinal injury and the neonates with severe intestinal injury were(14.59±1.78)and

关 键 词：二胺氧化酶 肠脂肪酸 新生儿窒息 胃肠功能障碍 

分 类 号：R97[医药卫生—药品]