The application of digital holography for accurate three-dimensional localisation of mosquito-bednet interaction  

作　　者：Matthew L Hall Katherine Gleave Angela Hughes Philip J McCall Catherine E Towers David P Towers 

机构地区：[1]Optical Engineering Group,School of Engineering,University of Warwick,Coventry,CV47AL,UK [2]Vector Biology Department,Liverpool School of Tropical Medicine,Liverpool,L35QA,UK

出　　处：《Light(Advanced Manufacturing)》2022年第3期13-27,共15页光（先进制造）（英文）

基　　金：This work was supported by the Engineering and Physical Sciences Research Council under Grant Number EP/N509796/1.A UK Medical Research Council award(MRC:MR/M011941/1)also contributed to this work.

摘　　要：Understanding mosquito interaction with long-lasting insecticidal bednets is crucial in the development of more effective intervention methods to protect humans from malaria transmission.As such,a 240×240×1000 mm laboratory setup for the in-line recording of digital holograms and subsequent in-focus reconstruction and 3D localisation of mosquitoes is presented.Simple bednet background removal methods are used to accurately localise a mosquito obscured by a bednet in 3D coordinates.Simulations and physical data demonstrate that this method is suitable for mosquitoes positioned 3−1000 mm behind a bednet.A novel post-processing technique,involving a cascade-correlation of a Tamura of Intensity focus metric extracted from digitally reconstructed scenes,accurately localises mosquitoes positioned 35−100 mm behind a bednet from a single digital hologram.The result of this study is a scalable digital holographic methodology to examine mosquito-bednet interaction in 3D at a level of accuracy previously only seen in 2D imaging of mosquitoes in a much smaller volume.

关 键 词：Digital Holography METROLOGY ENTOMOLOGY 

分 类 号：R318[医药卫生—生物医学工程]