Spatially resolved transcriptomics:advances and applications  

作　　者：Honglin Duan Tao Cheng Hui Cheng 

机构地区：[1]State Key Laboratory of Experimental Hematology,Haihe Laboratory of Cell Ecosystem,National Clinical Research Center for Blood Diseases,Institute of Hematology and Blood Diseases Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin,China [2]Center for Stem Cell Medicine,Chinese Academy of Medical Sciences,Tianjin,China [3]Department of Stem Cell&Regenerative Medicine,Peking Union Medical College,Tianjin,China

出　　处：《Blood Science》2023年第1期1-14,共14页血液科学（英文）

基　　金：the Ministry of Science and Technology of China(2021YFA1100900 and 2020YFE0203000);the National Natural Science Foundation of China(81730006,81922002,81861148029,and 81870086);CAMS Innovation Fund for Medical Sciences(2021-I2M-1-040 and 2021-I2M-1-019);Haihe Laboratory of Cell Ecosystem Innovation Fund(HH22KYZX0016);Distinguished Young Scholars of Tianjin(19JCJQJC63400).

摘　　要：Spatial transcriptomics,which is capable of both measuring all gene activity in a tissue sample and mapping where this activity occurs,is vastly improving our understanding of biological processes and disease.The field has expanded rapidly in recent years,and the development of several new technologies has resulted in spatially resolved transcriptomics(SRT)becoming highly multiplexed,high-resolution,and high-throughput.Here,we summarize and compare the major methods of SRT,including imagingbased methods,sequencing-based methods,and in situ sequencing methods.We also highlight some typical applications of SRT in neuroscience,cancer biology,developmental biology,and hematology.Finally,we discuss future possibilities for improving spatially resolved transcriptomic methods and the expected applications of such methods,especially in the adult bone marrow,anticipating that new developments will unlock the full potential of spatially resolved multi-omics in both biological research and the clinic.

关 键 词：In situ hybridization In situ sequencing Spatially resolved multi-omics Spatially resolved transcriptomics 

分 类 号：Q78[生物学—分子生物学]