Nano-selenium alleviates cadmium-induced cerebellar injury by activating metal regulatory transcription factor 1 mediated metal response  

作　　者：Shao-Shuai Bi Milton Talukder Hai-Tao Jin Mei-Wei Lv Jing Ge Cong Zhang Jin-Long Li 

机构地区：[1]College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China [2]College of Biotechnology and Pharmaceutical Engineering of West Anhui University,Lu’an 237012,China [3]Department of Physiology and Pharmacology,Faculty of Animal Science and Veterinary Medicine,Patuakhali Science and Technology University,Barishal,8210,Bangladesh [4]Quality and Safety Institute of Agricultural Products,Heilongjiang Academy of Agricultural Sciences,Harbin 150010,China [5]College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China [6]Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment,Northeast Agricultural University,Harbin 150030,China [7]Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine,Northeast Agricultural University,Harbin 150030,China

出　　处：《Animal Nutrition》2022年第4期402-412,共11页动物营养（英文版）

基　　金：National Natural Science Foundation of China(No.32172932 and No.32102739);Key Program of Natural Science Foundation of Heilongjiang Province of China(No.ZD2021C003);High-level Talent Scientific Research Start-up Foundation of Wanxi University(No.WGKQ2022031);China Agricultural Research System of MOF and MARA(No.CARS35);Distinguished Professor of Longjiang Scholars Support Project(No.T201908);Outstanding Talents of Henan Agricultural University(Grant No.30500997);Project funded by China Postdoctoral Science Foundation(2021M690925);Heilongjiang Touyan Innovation Team Program。

摘　　要：This study aims to investigate the role of metal regulatory transcription factor 1(MTF1)-mediated metal response in cadmium(Cd)-induced cerebellar injury,and to evaluate the antagonistic effects of nanoselenium(Nano-Se)against Cd toxicity.A total of 80 chicks(1 d old,male,Hy-Line Variety White)were randomly allocated to 4 treatment groups for 3 months:the control group(fed with a basic diet,n=20),the Nano-Se group(basic diet with 1 mg/kg nano-Se 1 mg/kg Nano-Se in basic diet,n=20),the NanoSe+Cd group(basic diet with 1 mg/kg Nano-Se and 140 mg/kg Cd Cl_(2),n=20)and the Cd group(basic diet with 140 mg/kg Cd Cl_(2),n=20).The results of the experiment showed that the Purkinje cells were significantly decreased with their degradation and indistinct nucleoli after Cd exposure.Moreover,exposure to Cd caused a significant accumulation of Cd and cupper.However,the contents of Se,iron,and zinc were decreased,thereby disturbing the metal homeostasis in the cerebellum.The Cd exposure also resulted in high levels of malondialdehyde(MDA)and down regulation of selenoprotein transcriptome.Furthermore,the expressions of MTF1,metallothionein 1(MT1),MT2,zinc transporter 3(ZNT3),ZNT5,ZNT10,zrt,irt-like protein 8(ZIP8),ZIP10,transferrin(TF),ferroportin 1(FPN1),ATPase copper transporting beta(ATP7B),and copper uptake protein 1(CTR1)were inhibited by Cd exposure.However,all these changes were significantly alleviated by the supplementation of Nano-Se.This study proved that Cd could disorder metal homeostasis and induce oxidative stress,whereas Nano-Se could relieve all these negative effects caused by Cd via activating the MTF1-mediated metal response in the cerebellum of chicken.

关 键 词：NANO-SELENIUM CADMIUM Selenoprotein transcriptome MTF1-mediated metal response Cerebellar injury 

分 类 号：S859.8[农业科学—临床兽医学]