Tetrapyrrole biosynthesis pathway regulates plastid-to-nucleus signaling by controlling plastid gene expression in plants  

作　　者：Yunlong Wang Yihua Wang Xiaopin Zhu Yulong Ren Hui Dong Erchao Duan Xuan Teng Huanhuan Zhao Rongbo Chen Xiaoli Chen Jie Lei Hang Yang Yunlu Tian Liangming Chen Xi Liu Shijia Liu Ling Jiang Haiyang Wang Jianmin Wan 

机构地区：[1]State Key Laboratory for Crop Genetics and Germplasm Enhancement,Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University,Nanjing 210095,P.R.China [2]National Key Facility for Crop Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China

出　　处：《Plant Communications》2023年第1期1-12,共12页植物通讯（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(91935301);National Natural Science Foundation of China Joint Program(U1701232);Jiangsu Science and Technology Development Program(BE2021360);Jiangsu Agricultural Science and Technology Innovation Fund Project(SCX(19)1079);Jiangsu Province Agriculture Independent Innovation Fund Project(CX(19)1002);National Key Research and Development Program of China(2016YFD0100903);the Fundamental Research Funds for the Central Universities(JCQY201902).

摘　　要：Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast developmental status and is essential for the photoautotrophic lifestyle of plants.Previous studies have established that tetrapyrrole biosynthesis(TPB)and plastid gene expression(PGE)play essential roles in plastid retrograde signaling during early chloroplast biogenesis;however,their functional relationship remains unknown.In this study,we generated a series of rice TPB-related gun(genome uncoupled)mutants and systematically analyzed their effects on nuclear and plastid gene expression under normal conditions or when subjected to treatments with norflurazon(NF;a noncompetitive inhibitor of carotenoid biosynthesis)and/or lincomycin(Lin;a specific inhibitor of plastid translation).We show that under NF treatment,expression of plastid-encoded polymerase(PEP)-transcribed genes is significantly reduced in the wild type but is derepressed in the TPB-related gun mutants.We further demonstrate that the derepressed expression of PEPtranscribed genes may be caused by increased expression of the PEP core subunit and nuclear-encoded sigma factors and by elevated copy numbers of plastid genome per haploid genome.In addition,we show that expression of photosynthesis-associated nuclear genes(PhANGs)and PEP-transcribed genes is correlated in the rice TPB-related gun mutants,with or without NF or Lin treatment.A similar correlation between PhANGs and PGE is also observed in the Arabidopsis gun4 and gun5 mutants.Moreover,we show that increased expression of PEP-transcribed plastid genes is necessary for the gun phenotype in NF-treated TPB-related gun mutants.Further,we provide evidence that these TPB-related GUN genes act upstream of GUN1 in the regulation of retrograde signaling.Taken together,our results suggest that the TPB-related GUN genes control retrograde plastid signaling by regulating the PGE-dependent retrograde signaling pathway.

关 键 词：tetrapyrrole biosynthesis plastid gene expression plastid retrograde signal 

分 类 号：Q943.2[生物学—植物学]