芒果叶提取物体外抗HIV-1活性研究  

A study on the anti-HIV-1 activity of Mango(Mangifera indica L.)leaf extract in vitro

作　　者：王倩 叶力 王捷[3] 周波 覃秋珍 詹妤婕 刘欣 刘洁 梁浩 WANG Qian;YE Li;WANG Jie(Guangxi Key Laboratory of Acquired Immune Deficiency Syndrome(AIDS)Prevention and Treatment,Life Science Institute,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学生命科学研究院,广西艾滋病防治研究重点实验室,南宁530021 [2]广西医科大学公共卫生学院 [3]广西医科大学制药厂

出　　处：《中国临床新医学》2023年第1期40-44,共5页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：国家自然科学基金资助项目(编号:81860655);广西科技计划项目(编号:桂科AB19245038);广西高校中青年教师科研基础能力提升项目(编号:2022KY0090)。

摘　　要：目的探讨芒果叶提取物的体外抗人类免疫缺陷病毒1(HIV-1)活性,为研究与开发传统中医药资源治疗获得性免疫缺陷综合征(AIDS)提供参考。方法通过三磷酸腺苷(ATP)法评估干预药物对TZM-bl细胞和MT-2细胞活性的影响。构建TZM-bl-HIV-1_(ⅢB)、MT-2-HIV-1_(ⅢB)两种细胞-病毒感染模型,通过荧光素酶活性检测试剂检测病毒活性,评估干预药物的抗HIV-1活性。观察MT-2-HIV-1_(ⅢB)细胞系统中芒果叶提取物对细胞病变效应(CPE)的抑制情况。计算芒果叶提取物的半数毒性浓度(CC_(50))、半数抑制浓度(IC_(50))和选择指数(SI)。结果在TZM-bl-HIV-1_(ⅢB)和MT-2-HIV-1_(ⅢB)两种细胞-病毒感染模型中,芒果叶提取物均显示出抗HIV-1活性,且呈现剂量依赖性。在TZM-bl-HIV-1_(ⅢB)模型中,芒果叶提取物的CC_(50)为(320.00±29.44)μg/ml,IC_(50)为(8.86±0.26)μg/ml,SI为36.14。在MT-2-HIV-1_(ⅢB)模型中,芒果叶提取物CC_(50)为(174.13±22.36)μg/ml,IC_(50)为(15.23±9.99)μg/ml,SI为11.44。结论芒果叶提取物的体外细胞毒性小,抗HIV-1活性显著,具有潜在的开发利用价值。Objective To investigate the in-vitro activity of Mango(Mangifera indica L.)leaf extract against human immunodeficiency virus 1(HIV-1),and to provide reference for the research and development of Traditional Chinese Medicine resources for the treatment of acquired immune deficiency syndrome(AIDS).Methods The effects of intervention drugs on the activity of TZM-bl cells and MT-2 cells were evaluated by adenosine triphosphate(ATP)assay.Two cell-virus infection models of TZM-bl-HIV-1_(ⅢB)and MT-2-HIV-1_(ⅢB)were constructed,and the virus activity was detected by luciferase activity detection reagent,so as to evaluate the anti-HIV-1 activity of intervention drugs.The inhibition of Mango leaf extract on cytopathic effect(CPE)in MT-2-HIV-1_(ⅢB)cell system was observed.Half toxic concentration(CC_(50)),half inhibitory concentration(IC_(50))and selection index(SI)of Mango leaf extract were calculated.Results In the TZM-bl-HIV-1_(ⅢB)and MT-2-HIV-1_(ⅢB)cell-virus infection models,Mango leaf extract showed anti-HIV-1 activity in a dose-dependent manner.In the TZM-bl-HIV-1_(ⅢB)model,the CC_(50),IC_(50)and SI of Mango leaf extract were(320.00±29.44)μg/ml,(8.86±0.26)μg/ml and 36.14,respectively.In the MT-2-HIV-1_(ⅢB)model,the CC_(50),IC_(50)and SI of Mango leaf extract were(174.13±22.36)μg/ml,(15.23±9.99)μg/ml and 11.44,respectively.Conclusion Mango leaf extract has low cytotoxicity in vitro and significant anti-HIV-1 activity,which has potential development and utilization value.

关 键 词：芒果叶提取物 细胞毒性 抗HIV-1活性 

分 类 号：R285.5[医药卫生—中药学]