鱼腥草提取物对大鼠肺炎克雷伯菌肺炎的干预效果  

Interventional effect of houttuynia cordata extract on rats with Klebsiella pneumoniae pneumonia

作　　者：王晓鑫[1] 白静[1] 任智慧 吴巍[1] 冯霞[1] WANG Xiao-xin;BAI Jing;REN Zhi-hui;WU Wei;FENG Xia(Affiliated Hospital of Inner Mongolia Medical University,Hohhot,Inner Mongolia O1005O,China)

机构地区：[1]内蒙古医科大学附属医院急诊科,内蒙古呼和浩特010050

出　　处：《中华医院感染学杂志》2022年第22期3375-3379,共5页Chinese Journal of Nosocomiology

基　　金：内蒙古医科大学青年创新基金资助项目(YKD2018QNCX102)。

摘　　要：目的分析鱼腥草提取物对大鼠肺炎克雷伯菌肺炎的干预效果。方法选取SD大鼠120只,分为对照组20只,感染组34只,感染+低剂量组33只,感染+高剂量组33只进行建模,将感染组、感染+低剂量组、感染+高剂量组建模成功30只大鼠进行灌胃,观察大鼠体质量、肺质量、肺湿质量指数、死亡关联蛋白激酶1(DAPK1)表达量、干扰素-γ(IFN-γ)、白细胞介素-6(IL-6)表达水平、Toll样受体4(TLR4)、核转录因子κB(NF-κB)蛋白相对表达量。结果与对照组相比,感染组、感染+低剂量组、感染+高剂量组体质量、肺质量降低,肺湿质量指数、DAPK1表达量、IFN-γ、IL-6、TLR4、NF-κB蛋白相对表达量升高(P<0.05);与感染组相比,感染+低剂量组、感染+高剂量组体质量、肺质量升高,肺湿质量指数、DAPK1表达量、IFN-γ、IL-6、TLR4、NF-κB蛋白相对表达量降低(P<0.05);与感染+低剂量组相比,感染+高剂量组体质量、肺质量升高,肺湿质量指数、DAPK1表达量、IFN-γ、IL-6、TLR4、NF-κB蛋白相对表达量降低(P<0.05)。结论鱼腥草提取物可使肺炎肺部感染大鼠DAPK1、炎症因子降低,其作用机制可能与TLR4/NF-κB通路有关。OBJECTIVE To observe the interventional effect of houttuynia cordata extract on rats with Klebsiella pneumoniae pneumonia.METHODS Totally 120 SD rats were enrolled in the study and divided into the control group with 20 rats,the infection group with 34 rats,the infection plus low-dose group with 33 rats and the infection plus high-dose group with 33 rats.The rat models were established,the rat models of 30 rats that were established successfully in the infection group,the infection plus low-dose group and the infection plus high-dose group were treated with gavage.The body weight,lung weight,lung wet mass index,expression level of death-associated protein kinase 1(DAPK1),expression levels of interferon-γ(IFN-γ)and interleukin-6(IL-6)and the relative expression levels of Toll-like receptor 4(TLR4)and nuclear transcription factor-κB(NF-κB)were observed.RESULTS The body weight and lung weight of the infection group,the infection plus low-dose group and the infection plus high-dose group were less than those of the control group;while the lung wet mass index,DAPK1 expression level and relative expression levels of IFN-γ,IL-6,TLR4 and NF-κB of the infection group,the infection plus low-dose group and the infection plus high-dose group were higher than those of the control group(P<0.05).The body weight and lung weight of the infection plus low-dose group and the infection plus high-dose group were greater than those of the infection group,while the lung wet mass index,DAPK1 expression level and relative expression levels of IFN-γ,IL-6,TLR4 and NF-κB of the infection plus low-dose group and the infection plus high-dose group were lower than those of the infection group(P<0.05).The body weight and lung weight of the infection plus high-dose group were greater than those of the infection plus low-dose group,while the lung wet mass index,DAPK1 expression level and relative expression levels of IFN-γ,IL-6,TLR4 and NF-κB of the infection plus high-dose group were lower than those of the infection plus low-dose grou

关 键 词：肺炎 肺部感染 鱼腥草提取物 死亡关联蛋白激酶1 作用机制 

分 类 号：R378.996[医药卫生—病原生物学]