IL-10、TLR9基因多态性与儿童EB病毒感染的关联  

Association of IL-10 and TLR9 gene polymorphisms with EB virus infection in children

作　　者：李革[1] 黄永建[2] 陈放 余怡如 刘兴楼 LI Ge;HUANG Yong-jian;CHEN Fang;YU Yi-ru;LIU Xing-lou(Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院儿科病毒研究室,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院儿科,湖北武汉430030

出　　处：《中华医院感染学杂志》2022年第22期3472-3476,共5页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金面上基金资助项目(81301425)。

摘　　要：目的探究白细胞介素-10(IL-10)、Toll样受体9(TLR9)基因多态性与儿童EB病毒(EBV)感染的关联。方法选择2018年5月-2019年4月华中科技大学同济医学院附属同济医院收治的EBV感染患儿368例,其中EBV感染所致传染性单核细胞增多症(IM)128例(IM组)、单纯性EBV感染240例(单纯性EBV组),并选择同期未感染EBV的健康儿童100名为对照组,应用聚合酶链式扩增反应检测所有儿童外周血中IL-10基因(rs1800896、rs1800871、rs1800872位点)、TLR9基因(rs187084、rs352139、rs1352140位点)的基因多态性,分析各单核苷酸多态性(SNP)的基因型频率、等位基因频率在三组儿童中的分布差异。结果三组儿童IL-10、TLR9基因共6个位点均符合Hardy-Weinberg平衡(P>0.05);IL-10-rs1800896、IL-10-rs1800871、IL-10-rs1800872位点和TLR9-rs187084、TLR9-rs352139、TLR9-rs1352140位点各基因型及等位基因分布在IM组、单纯性EBV组、对照组儿童间比较,无统计学差异,其中,IM组、单纯性EBV组和对照组儿童IL-10-rs1800896位点AG型变异率低,分别为9.38%、8.33%、14.00%,且均无GG基因型,G等位基因频率最低,分别为4.69%、4.17%、7.00%。结论IL-10-rs1800896、IL-10-rs1800871、IL-10-rs1800872位点和TLR9-rs187084、TLR9-rs352139、TLR9-rs1352140位点的多态性与儿童EBV感染的易感性无关联。OBJECTIVE To explore the association of gene polymorphisms of interleukin-10(IL-10)and Toll-like receptor 9(TLR9)with Epstein-Barr virus(EBV)infection in children.METHODS A total of 368 children with EBV infection who were treated in Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology from May 2018 to Apr 2019 were enrolled in the study,128 of whom had infectious mononucleosis(IM)caused by EBV infection and were assigned as the IM group,240 had simple EBV infection and were assigned as the simple EBV group.Meanwhile,100 healthy children who did not have EBV infection were chosen as the control group.The polymorphisms of IL-10 gene(rs1800896 locus,rs1800871 locus,rs1800872 locus)and TLR9 gene(rs187084 locus,rs352139 locus,rs1352140 locus)in peripheral blood of all the children were detected by polymerase chain amplification reaction.The frequencies of genotypes and alleles of single nucleotide polymorphism(SNP)were observed and compared among the three groups of children.RESULTS All of the 6 loci of IL-10 and TLR9 gene were in line with Hardy-Weinberg balance(P>0.05).There were no significant differences in the genotypes and alleles at IL-10-rs1800896,IL-10-rs1800871,IL-10-rs1800872 loci and TLR9-rs187084,TLR9-rs352139 and TLR9-rs1352140 loci among the IM group,the simple EBV group and the control group.The mutation rates of AG genotype at IL-10-rs1800896 locus of the IM group,the simple EBV group and the control group were respectively 9.38%,8.33%and 14.00%,no GG genotype was found,and the frequencies of G allele were 4.69%,4.17%and 7.00%,respectively.CONCLUSION There is no association between the polymorphisms at IL-10-rs1800896,IL-10-rs1800871,IL-10-rs1800872,TLR9-rs187084,TLR9-rs352139 and TLR9-rs1352140 loci and the susceptibility to EBV infection.

关 键 词：白细胞介素-10 TOLL样受体9 基因多态性 EB病毒 儿童 关联 

分 类 号：R725.1[医药卫生—儿科]