ADRB1基因多态性与EV71感染手足口病严重程度的关联  

Relationship between ADRB1 gene polymorphisms and severity of EV71-infected hand-foot-and-mouth disease

作　　者：付文静 盛爱芹 鲍菁 叶菲 葛敏娜 FU Wen-jing;SHENG Ai-qin;BAO Jing;YE Fei;GE Min-na(Children's Hospital,School of Medicine,Zhejiang University,Hangzhou,Zhejiang 310003,China)

机构地区：[1]浙江大学医学院附属儿童医院呼吸/神经内科,浙江杭州310003 [2]浙江大学医学院附属儿童医院内科,浙江杭州310003 [3]浙江大学医学院附属儿童医院肾内科,浙江杭州310003

出　　处：《中华医院感染学杂志》2022年第23期3653-3657,共5页Chinese Journal of Nosocomiology

基　　金：浙江省科研基金资助项目(2020K021)。

摘　　要：目的分析β1肾上腺素能受体(ADRB1)基因多态性与肠道病毒71型(EV71)感染手足口病(HFMD)严重程度的关系。方法选择医院2018年2月-2020年10月收治的100例EV71感染HFMD患儿为HFMD组,根据病情严重程度分为轻症组(n=41)、重症组(n=32)、危重症组(n=27),另选同期35名健康体检儿童为对照组,聚合酶链式反应(PCR)技术检测ADRB1基因单核苷酸多态性位点Ser49Gly、Arg389Gly的多态性,分析ADRB1基因多态性与EV71感染HFMD严重程度的关系。结果ADRB1基因Ser49Gly、Arg398Gly位点的基因型分布均符合Hardy-Weinberg遗传平衡定律;HFMD组Arg398Gly位点CC、CG、GG基因型频率,C、G等位基因频率与对照组比较,差异有统计学意义(P<0.05);HFMD患儿轻症组、重症组、危重症组ADRB1基因Ser49Gly位点AA、AG、GG基因型频率及A、G等位基因频率比较,差异无统计学意义,Arg398Gly位点CC、CG、GG基因型频率及C、G等位基因频率比较,差异有统计学意义(P<0.05);Logistic回归分析可知,ADRB1基因Arg398Gly位点CG基因频率与HFMD重症发生有关(P<0.05)。结论ADRB1基因Arg398Gly位点基因多态性与EV71感染的HFMD发生率有关,其中G等位基因可增加EV71的感染风险及加重病情。OBJECTIVE To analyze the relationship betweenβ1 adrenergic receptor(ADRB1)gene polymorphism and severity of enterovirus 71(EV71)-infected hand-foot-and-mouth disease(HFMD).METHODS Total of 100 children with EV71-infected HFMD treated in the hospital between Feb 2018 and Oct 2020 were selected as the HFMD group.According to the severity of disease,the patients were divided into the mild group(n=41),severe group(n=32)and critically ill group(n=27).Another 35 healthy children under physical examination during the same period were enrolled as the control group.The polymorphisms of single nucleotide polymorphism loci Ser49Gly and Arg389Gly of ADRB1 gene were detected by polymerase chain reaction(PCR),and the relationship between ADRB1 gene polymorphisms and severity of EV71-infected HFMD was analyzed.RESULTS The genotype distribution at Ser49Gly and Arg398Gly loci of ADRB1 gene conformed to Hardy-Weinberg genetic equilibrium law.The differences in the frequencies of CC,CG,GG genotypes,C and G alleles of Arg398Gly between the HFMD group and control group were significant(P<0.05).The differences in frequencies of AA,AG and GG genotypes and frequencies of A and G alleles at Ser49Gly locus of ADRB1 gene among children with HFMD in mild group,severe group and critically ill group were not significant,while the differences in the frequencies of CC,CG and GG genotypes and frequencies of C and G alleles at Arg398Gly locus were significant(P<0.05).Multivariate Logistics regression analysis showed that the frequency of CG genotype at Arg398Gly locus of ADRB1 gene was related to the occurrence of severe HFMD(P<0.05).CONCLUSION The gene polymorphism at Arg398Gly locus of ADRB1 gene is related to the incidence rate of EV71-infected HFMD.The G allele can increase the risk of EV71 infection and aggravate the disease.

关 键 词：Β1肾上腺素能受体 肠道病毒71型 手足口病 基因多态性 基因频率 等位基因 

分 类 号：R512.5[医药卫生—内科学]