紫荆属种间杂交胚拯救研究  

Study on embryo rescue of interspecific hybridization in Cercis Linn.

作　　者：杨莹 于淑霞 杨林毅 赵嘉欣 刘弢 张玉焕 李江 赵玉荣 刘双枝 张林 YANG Ying;YU Shuxia;YANG Linyi;ZHAO Jiaxin;LIU Tao;ZHANG Yuhuan;LI Jiang;ZHAO Yurong;LIU Shuangzhi;ZHANG Lin(Yanling Zhonglin Garden Engineering Co.,Ltd.,Xuchang Key Laboratory of Bauhinia Germplasm Innovation and Utilization,Xuchang,Henan 461200,China;Henan Spring Garden Landscape Engineering Co.,Ltd.,Zhengzhou 450016,China;Henan Giant Bauhinia Cultivation Engineering Technology Research Center,Xuchang,Henan 461200,China;Garden Water Affairs Bureau,Zhengdong New District,Zhengzhou City,Zhengzhou 450006,China)

机构地区：[1]鄢陵中林园林工程有限公司,许昌市紫荆属种质创新与利用重点实验室,河南许昌461200 [2]河南四季春园林艺术工程有限公司,郑州450016 [3]河南省巨紫荆栽培工程技术研究中心,河南许昌461200 [4]郑州市郑东新区园林水务局,郑州450006

出　　处：《植物生理学报》2022年第11期2173-2180,共8页Plant Physiology Journal

基　　金：许昌市优良紫荆属种质资源创新及产业化项目(20200112008)。

摘　　要：为克服紫荆属(Cercis Linn.)种间杂交种子后期发育障碍,提高杂交后代成活率,缩短育种周期,本实验以紫荆属的湖北紫荆和紫荆杂交的未成熟胚为外植体材料,研究不同消毒方式、不同胚龄对杂交胚萌发的影响以及筛选最适诱导、增殖及生根培养基。结果表明:消毒方式用体积分数为0.1%的HgCl2溶液处理5 min的效果最佳;存活率为76.67%。在授粉后120 d时采样,胚的萌发率(66.3%)最高。胚拯救最适宜的诱导培养基:MS+3.0 mg·L^(-1)6-BA+0.05 mg·L^(-1)NAA+0.05 mg·L^(-1)GA+150 mg·L^(-1)AC,诱导率为95%;最佳增殖培养基:MS+1.5 mg·L^(-1)KT+1.5 mg·L^(-1)6-BA+0.05 mg·L^(-1)NAA+150 mg·L^(-1)AC,增殖系数为4.79;最佳生根培养基:1/2MS+0.5 mg·L^(-1)NAA+0.1 mg·L^(-1)VH+150 mg·L^(-1)AC,生根率为81.67%。下地炼苗后的移栽成活率为87.53%。本实验旨在建立有效的胚拯救体系,为提高紫荆属种间远缘杂交成功率提供有效技术,同时为紫荆属种间杂交育种提供新的种质资源。In order to overcome the late developmental obstacles of interspecific hybrid seeds of Cercis Linn.,increase the survival rate of interspecific hybrids of Cercis Linn.and shorten the breeding cycle,immature embryos of C.glabra×C.chinensis were used to investigate the effect of different disinfection methods and different embryo ago on the germination of hybrid embryos and to choose out the optimun induction,proliferation and rooting media.The results showed that the best disinfection method was to disinfect the seed for 5 min with 0.1%HgCl2,which the survival rates were 76.67%.The optimal sampling times were 120 days after pollination,with the highest developed embryo rate(66.3%).MS+3.0 mg·L^(-1)6-BA+0.05 mg·L^(-1)NAA+0.05 mg·L^(-1)GA3+150 mg·L^(-1)AC was the best medium for embryo rescue,the induction rate of embryo was 95%.The optimal medium for proliferation was MS+1.5 mg·L^(-1)KT+1.5 mg·L^(-1)6-BA+0.05 mg·L^(-1)NAA+150 mg·L^(-1)AC,the proliferation coefficient was 4.79.And 1/2MS+0.5 mg·L^(-1)NAA+0.1mg·L^(-1)VH+150 mg·L^(-1)AC for rooting was best with the rate of 81.67%.The survival rate of transplanting after hardening in the ground was 87.53%.The purpose of this experiment is to establish an effective embryo rescue system,to provide an effective technology for improving the success rate of distant hybridization among Cercis Linn.,and to provide new germplasm resources for Cercis Linn.interspecific hybrid breeding.

关 键 词：紫荆属 种间杂交 胚龄 胚拯救 

分 类 号：S685.99[农业科学—观赏园艺]