谷子SNF1-related kinase1(SnRK1)基因的全基因组鉴定及其在糖信号和非生物胁迫下的表达特性分析  

Genome-wide identification of SNF1-related kinase1(SnRK1)genes in Setaria italica and analysis of its expression characteristics responding to sugar signal and abiotic stress

作　　者：赵耀飞 韩彦卿 侯书培 刘赵文 韩渊怀[1,2] 李雅琼 ZHAO Yaofei;HAN Yanqing;HOU Shupei;LIU Zhaowen;HAN Yuanhuai;LI Yaqiong(College of Agriculture,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China;Shanxi Key Laboratory of Minor Crop Germplasm Innovation and Molecular Breeding,Jinzhong,Shanxi 030801,China;College of Plant Protection,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China)

机构地区：[1]山西农业大学农学院,山西晋中030801 [2]杂粮种质创新与分子育种山西省重点实验室,山西晋中030801 [3]山西农业大学植物保护学院,山西晋中030801

出　　处：《植物生理学报》2022年第11期2191-2208,共18页Plant Physiology Journal

基　　金：山西省优秀博士来晋工作奖励资金科研项目(SXYBKY2019038和SXYBKY2019035);山西农业大学科技创新基金(2020BQ06和2016YJ06)

摘　　要：蔗糖非酵解型蛋白激酶1(SnRK1)是植物糖信号途径中的关键因子,参与了植物生长发育的调节。然而在谷子中尚无有关SnRK1的报道。研究谷子中的SnRK1基因在能量代谢及响应非生物胁迫中表达模式,能够为揭示谷子(Setaria italica)生长发育及逆境响应调控过程奠定基础。我们在谷子基因组中鉴定到10个SnRK1基因家族成员(SiSnRK1)。系统进化树分析显示SiSnRK1与同为C4植物的高粱和玉米中的SnRK1亲缘关系较近,并且其含有SnRK1家族成员保守的结构域。SiSnRK1α1、SiSnRK1α2、SiSnRK1β1、SiSnRK1β2、SiSnRK1β4、SiSnRK1βγ1和SiSnRK1βγ2的表达量会在黑暗处理后显著提高,且在蔗糖处理后显著下降。而除SiSnRK1α4外,其余九个基因在干旱、高盐、机械损伤和ABA处理后均出现一定程度的上调表达,但上调速度略有不同,其中SiSnRK1α3、SiSnRK1β4和SiSnRK1βγ1在多种非生物胁迫条件下均表现出类似的表达模式。上述结果表明谷子中多个SnRK1基因在糖信号途径以及非生物胁迫响应中可能发挥着重要作用,其中,SiSnRK1α3、SiSnRK1β4和SiSnRK1βγ1在多种非生物胁迫处理中表现出类似的表达模式,它们可能协同调控谷子响应逆境胁迫相关反应。SNF1-related kinase1(SnRK1)are key factors in the plant sugar signaling pathway,participating in the regulation of plant growth and development.However,there is no report about SnRK1 genes in foxtail millet(Setaria italica).Exploring the expression pattern of Sn RK1 genes during energy metabolism and responses to abiotic stress can lay a foundation for revealing the regulation mechanism of growth and development and stress response in foxtail millet.We identified 10 members of the SiSnRK1 in the genome of foxtail millet.Phylogenetic tree analysis showed that SiSnRK1 was closely related to SnRK1 in sorghum and maize,which were also C4 plants.SiSnRK1 family members contained conserved domains of SnRK1s in other species.The expression levels of Si Sn RK1α1,SiSnRK1α2,SiSnRK1β1,SiSnRK1β2,SiSnRK1β4,SiSnRK1βγ1 and Si Sn RK1βγ2 were significantly increased after dark treatment,and significantly decreased after sucrose treatment.Except Si Sn RK1α4,the other nine genes were upregulated after drought,salt,mechanical injury and ABA treatments,but the upregulated time was slightly different.Si Sn RK1α3,SiSnRK1β4 and Si SnRK1βγ1 showed similar expression patterns under various abiotic stress treatments.These results showed that multiple Sn RK1 genes in foxtail millet may play important roles in the sugar signaling pathway and responses to abiotic stress.As Si Sn RK1α3,Si Sn RK1β4 and Si Sn RK1βγ1 showed similar expression patterns in multiple stress responses,the genes may coordinatively regulate stress resistance in foxtail millet.

关 键 词：谷子 SnRK1 糖信号 非生物胁迫 表达模式 

分 类 号：S515[农业科学—作物学]