子宫内膜异位症患者的定量蛋白质组学分析  

Study on the pathogenesis of endometriosis by quantitative proteomics

作　　者：易金玲[1] 沈艳丽[1] 李菊清 腊晓琳[2] YI Jinling;SHEN Yanli;LI Juqing;LA Xiaoling(Department of Gynecology,the Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830011,China;Department of Reproduction and Pregnancy Assistance,the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830000,China)

机构地区：[1]新疆医科大学第五附属医院妇科,新疆乌鲁木齐830011 [2]新疆医科大学第一附属医院生殖助孕科,新疆乌鲁木齐830000

出　　处：《医药前沿》2022年第32期16-18,22,共4页Journal of Frontiers of Medicine

摘　　要：目的:通过高分辨质谱仪对子宫内膜异位症患者进行定量蛋白质组学分析,探讨子宫内膜异位症的诊断标志物及治疗靶点。方法:选取2019年1月—2021年12月新疆医科大学第五附属医院收治的卵巢子宫内膜异位症患者,取患者异位子宫内膜(PPF组)、在位子宫内膜(PNP组)组织样本各5例,再选取健康女性正常子宫内膜(NPF组)组织样本5例为对照组。提取总蛋白经蛋白酶解后,LC-MS高分辨质谱分析,TMT标记,肽段分级,高分辨质谱仪检测,生物信息学分析得出差异表达蛋白。结果:共鉴定到7575个差异蛋白,PPF组与NPF组差异蛋白296个,PNP组与NPF组差异蛋白52个,PPF组与PNP组差异蛋白535个。KEGG注释分析三组均筛选到且排名靠前的通路是PI3K-AKT信号通路。结论:定量蛋白质组学技术能有效筛选出子宫内膜异位症患者的差异蛋白,生物学分析筛选出有研究价值的蛋白靶点和代谢通路,为子宫内膜异位症患者新的诊断标志物及治疗靶点研究提供新思路。Objective To explore new diagnostic markers and therapeutic targets for patients with endometriosis by quantitative proteomics study with high resolution mass spectrometry.Methods Patients with ovarian endometriosis in the Fifth Affiliated Hospital of Xinjiang Medical University from January 2019 to December 2021 were selected as the research objects.Tissue samples of ectopic endometrium(PPF group)and eutopic endometrium(PNP group)were collected from 5 patients,and 5 tissue samples of normal endometrium from healthy women(NPF group)were selected as the control group.The total protein was extracted and digested by protease,followed by LC-MS/MS high resolution mass spectrometry,TMT labeling,peptide classification,high resolution mass spectrometry,and bioinformatics analysis to obtain differentially expressed proteins.Results A total of 7575 differential proteins were identified,including 296 in PPF group and NPF group,52 in PNP group and NPF group and 535 in PPF group and PNP group.KEGG annotation analysis showed that the PI3K-AKT signaling pathway ranked high among all the three groups.Conclusions Quantitative proteomics technology can effectively screen out the differentially expressed proteins in patients with endometriosis,and biological analysis can screen out protein targets and metabolic pathways with research value,which provides new ideas for the study of new diagnostic markers and therapeutic targets for patients with endometriosis.

关 键 词：子宫内膜异位症 蛋白质组学 串联质量标签 

分 类 号：R711.71[医药卫生—妇产科学]