鲁中地区矽肺患者血清1,25-二羟维生素D3、IL-6的检测及意义  

Detection and significance of serum 1,25-dihydroxyvitamin D3 and IL-6 in patients with silicosis in central Shandong

作　　者：张素华[1] 张红香[1] ZHANG Su-hua;ZHANG Hong-xiang(Department of Clinical Laboratory,Zibo Occupational Disease Prevention and Treatment Hospital,Zibo Shandong,255000,China)

机构地区：[1]淄博市职业病防治院检验科,山东淄博255000

出　　处：《职业与健康》2022年第23期3178-3181,共4页Occupation and Health

基　　金：中华国际科学交流基金会检验检测科技专项基金(Z2020LSD012)。

摘　　要：目的了解鲁中地区矽肺患者血清1,25-二羟维生素D_(3)[1,25-(OH)_(2)D_(3)]、白介素-6(IL-6)水平变化及意义。方法选取2020年11月—2021年2月鲁中地区淄博市职业病防治院住院的57例矽肺患者(矽肺壹、贰、叁期分别为32、18、7例),另选同期30名未接尘健康检查人员为对照组,采用高效液相色谱-串联质谱法检测血清1,25-(OH)_(2)D_(3)水平,电化学发光免疫分析法检测血清中IL-6水平。分析不同期别矽肺患者血清1,25-(OH)_(2)D_(3)、IL-6含量、肺功能的差异,分析二者与肺功能的相关性。结果矽肺壹、贰、叁期组血清1,25-(OH)_(2)D_(3)含量分别为(19.17±2.27)、(15.57±1.88)、(13.14±3.48)ng/mL,低于对照组[(22.51±2.75)ng/mL],差异均有统计学意义(均P<0.05);矽肺贰、叁期组1,25-(OH)_(2)D_(3)含量均低于壹期组,叁期组的含量低于贰期组(均P<0.05)。矽肺壹、贰、叁期组血清IL-6含量分别为(8.73±1.67)、(12.33±2.09)、(14.86±2.79)ng/mL,高于对照组[(4.76±1.66)ng/mL],差异均有统计学意义(均P<0.05)。矽肺贰期组血清IL-6含量高于壹期组,叁期组的含量高于贰期组,差异均有统计学意义(均P<0.05)。随着尘龄的增加,矽肺患者血清中1,25-(OH)_(2)D_(3)的含量逐渐降低,IL-6的含量逐渐增高,二者呈负相关(r=-0.336,P<0.05);矽肺组血清1,25-(OH)_(2)D_(3)含量与第1秒用力呼气量(forced expiratory volume in one second,FEV1)、第1秒用力呼气量占用力肺活量的比率(The ratio of forced expiratory volume in one second to forced vital capacity,FEV1/FVC)均呈正相关(r=0.443、0.305,均P<0.05),血清IL-6的含量与FEV1呈负相关(r=-0.317,P<0.05)。结论矽肺患者血清中1,25-(OH)_(2)D_(3)、IL-6含量的检测对了解矽肺的发生发展、肺功能评估有一定预测作用。Objective To investigate the changes and significance of serum 1,25-dihydroxyvitamin D_(3)[1,25-(OH)_(2)D_(3)]and interleukin-6(IL-6)in patients with silicosis in central Shandong.Methods From November 2020 to February 2021,57 patients with silicosis hospitalized in Zibo Occupational Disease Prevention and Control Hospital(32 patients with silicosis at the first stage,18 patients with silicosis at the second stage,and 7 patients with silicosis at the third stage)were collected,and 30 cases without dust exposure history ordered health check service during the same period were selected as the control group.The level of serum1,25-(OH)_(2)D_(3)was detected by high performance liquid chromatography-tandem mass spectrometry,and the level of serum IL-6was detected by electrochemiluminescence immunoassay.The differences in levels of serum 1,25-(OH)_(2)D_(3)and IL-6,lung function among silicosis patients in different stages,the correlation between them and lung function were analyzed.Results The levels of serum 1,25-(OH)_(2)D_(3)in the first,second and third stage of silicosis groups were(19.17±2.27),(15.57±1.88)and(13.14±3.48)ng/mL,respectively,which were lower than those in the control group[(22.51±2.75)ng/mL],and the differences were statistically significant(all P<0.05).The levels of serum 1,25-(OH)_(2)D_(3)in the second and third stage of silicosis groups were lower than those in the first stage group,and the levels in the third stage group were lower than those in the second stage group(all P<0.05).The levels of serum IL-6 in the first,second and third stage of silicosis groups were(8.73±1.67),(12.33±2.09)and(14.86±2.79)ng/mL,respectively,which were higher than those in the control group[(4.76±1.66)ng/mL],and the differences were statistically significant(all P<0.05).The levels of serum IL-6 in the second stage group were higher than those in the first stage group,while the levels in the third stage group were higher than those in the second stage group,and the differences were statistically significant(all

关 键 词：1 25-二羟维生素D3 白介素-6 矽肺 

分 类 号：R135.2[医药卫生—劳动卫生]