四川省514例新型冠状病毒感染者血清抗体水平分析  

Analysis of serum antibodies in 514 patients with COVID-19 in Sichuan

作　　者：杨慧萍[1] 韩媛媛 刘丽[1] 徐佳楠[1] 周丽君[1] 黄伟峰[1] 张韬[2] Yang Huiping;Han Yuanyuan;Liu Li;Xu Jianan;Zhou Lijun;Huang Weifeng;Zhang Tao(Institute of Microbiological Detection,Sichuan Province Center for Disease Control and Prevention,Chengdu 610041,China;West China School of Public Health,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川省疾病预防控制中心微生物检验所,成都610041 [2]四川大学华西公共卫生学院,成都610041

出　　处：《中华实验和临床病毒学杂志》2022年第6期649-653,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：四川省科技厅重点研发项目基金(2021YFQ0060);重庆市科技局资助项目(cstc2020jscx-cylhX0003);成都市科技局创新研发资助项目(2021-YF05-01585-SN)。

摘　　要：目的了解人感染新型冠状病毒(2019 novel coronavirus,2019-nCoV)后血清特异性抗体的变化规律。方法收集四川省514例新型冠状病毒肺炎(Coronavirus Disease 2019,COVID-19)确诊病例及无症状感染者(包括既往感染康复者、正在住院的病例和新病例)不同时间的血清样本1705份,采用直接化学发光免疫分析法检测血清中的IgM和IgG抗体。采用SPSS26.0对IgM和IgG抗体检测数据进行统计学分析,揭示IgM和IgG抗体的动态变化。结果研究共纳入确诊病例352人、无症状感染者162人。自确诊(或2019-nCoV核酸检测阳性)后8~149 d,92.75%~100%的感染者血清中IgG抗体呈阳性;持续至360 d后,仍有超过60%的感染者IgG抗体呈阳性,随时间增加,IgG抗体的S/CO值有下降趋势。连续观察15例感染者在确诊(或2019-nCoV核酸检测阳性)后8~14 d,血清中抗体阳性率达100%,120 d后IgM抗体转为阴性,随病程时间增加IgM抗体的S/CO值变化趋势明显。自确诊(或2019-nCoV核酸检测阳性)0~7 d和23~89 d时,确诊病例组与无症状感染者组IgM抗体阳性率差异均有统计学意义(P<0.05),至150 d后,确诊病例组的IgG抗体阳性率高于无症状感染者组,两者差异有统计学意义(χ^(2)=7.65,P=0.006)。结论不同类型的2019-nCoV感染者血清中IgM和IgG抗体水平随时间变化的规律不同。Objective To understand the changes of serum specific antibodies after human infection with 2019 novel coronavirus(2019-nCoV).Methods A total of 1705 serum samples from 514 confirmed cases and asymptomatic infected cases(including recovered,hospitalized,and newly infected cases)of Coronavirus Disease 2019(COVID-19)at different times were collected in Sichuan province.Chemiluminescence immunoassay(CLIA)was used to detect IgM and IgG antibodies.The variations of concentration of IgM and IgG were analyzed by statistical methods.SPSS26.0 was used for statistical analysis of IgM and IgG antibody detection data to reveal the dynamic changes of IgM and IgG antibodies.Results A total of 352 confirmed cases and 162 asymptomatic infected cases were included.92.75%-100%of infected cases were positive for IgG antibodies after 8-149 days from the date of diagnosis(or tested-positive for 2019-nCoV nucleic acid).More than 60%of the infected cases were still positive for IgG antibodies after 360 days.However,the signal-to-cutoff(S/CO)value of IgG antibodies decreased over time.Consecutive detecting serum of 15 infected cases,the positive rate of specific IgM and IgG antibodies was 100%in 8-14 days after diagnosis(or tested-positive for 2019-nCoV nucleic acid).IgM antibodies turned negative after 120 days.The S/CO value of IgM antibodies was observed to vary with disease course.During 0-7 days and 23-89 days after diagnosis(tested-positive for 2019-nCoV nucleic acid),there were statistically significant difference in the positive rate of IgM antibodies between confirmed cases and asymptomatic cases(P<0.05).After 150 days,the positive rate of IgG antibodies in confirmed cases group was higher than that in asymptomatic cases group.The difference is statistically significant(χ^(2)=7.65,P=0.006).Conclusions The changes of IgM and IgG antibodies levels in serum of different types of 2019-nCoV infected cases over time are different.

关 键 词：新型冠状病毒 新型冠状病毒肺炎 血清 抗体 

分 类 号：R446.6[医药卫生—诊断学] R563.1[医药卫生—临床医学]