Genomic and metabolomic analysis of Bacillus licheniformis with enhanced poly-γ-glutamic acid production through atmospheric and room temperature plasma mutagenesis  

作　　者：Xiaoyu Wei Lijie Yang Haiyan Wang Zhen Chen Yiyuan Xu Yue Weng Mingfeng Cao Qingbiao Li Ning He 

机构地区：[1]Department of Chemical and Biochemical Engineering,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China [2]College of Food and Biological Engineering,Jimei University,Xiamen 361021,China [3]College of Life Science,Xinyang Normal University,Xinyang 464000,China

出　　处：《Frontiers of Chemical Science and Engineering》2022年第12期1751-1760,共10页化学科学与工程前沿（英文版）

基　　金：This work was financially supported by the National Natural Science Foundation of China(Grant Nos.32170061 and 31871779).

摘　　要：Poly-γ-glutamic acid is an extracellular polymeric substance with various applications owing to its valuable properties of biodegradability,flocculating activity,water solubility,and nontoxicity.However,the ability of natural strains to produce poly-γ-glutamic acid is low.Atmospheric and room temperature plasma was applied in this study to conduct mutation breeding of Bacillus licheniformis CGMCC 2876,and a mutant strain M32 with an 11%increase in poly-γ-glutamic acid was obtained.Genome resequencing analysis identified 7 nonsynonymous mutations of ppsC encoding lipopeptide synthetase associated with poly-γ-glutamic acid metabolic pathways.From molecular docking,more binding sites and higher binding energy were speculated between the mutated plipastatin synthase subunit C and glutamate,which might contribute to the higher poly-γ-glutamic acid production.Moreover,the metabolic mechanism analysis revealed that the upregulated amino acids of M32 provided substrates for glutamate and promoted the conversion between L-and D-glutamate acids.In addition,the glycolytic pathway is enhanced,leading to a better capacity for using glucose.The maximum poly-γ-glutamic acid yield of 14.08 g·L^(–1)was finally reached with 30 g·L^(–1)glutamate.

关 键 词：ARTP mutagenesis Bacillus licheniformis poly-γ-glutamic acid metabolomics 

分 类 号：O62[理学—有机化学]