龙岩山麻鸭蛋品质性状的全基因组关联研究  

Genome-Wide Association Study of Egg Quality Traits in Longyan Shan-Ma Duck

作　　者：孙艳发[1,2] 吴琼 林如龙 陈红萍 甘秋云 沈玥 王亚茹 薛鹏飞 陈飞帆 刘健涛 周陈鑫 兰诗诗 潘浩哲 邓凡 岳稳 江宵兵 李焰 SUN YanFa;WU Qiong;LIN RuLong;CHEN HongPing;GAN QiuYun;SHEN Yue;WANG YaRu;XUE PengFei;CHEN FeiFan;LIU JianTao;ZHOU ChenXin;LAN ShiShi;PAN HaoZhe;DENG Fan;YUE Wen;JIANG XiaoBing;LI Yan(College of Life Sciences,Longyan University,Longyan 364012,Fujian;Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology/Fujian Provincial Universities Key Laboratory of Preventive Veterinary Medicine and Biotechnology/Longyan University,Longyan 364012,Fujian;Longyan Shan-Ma Duck Original Breeding Farm,Agricultural Bureau of Xinluo District,Longyan 364031,Fujian;Fujian Provincial Animal Husbandry Headquarters,Fuzhou 350003;College of Animal Science(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou 350002)

机构地区：[1]龙岩学院生命科学学院,福建龙岩364012 [2]龙岩学院/福建省家畜传染病防治与生物技术重点实验室/预防兽医学与生物技术福建省高校重点实验室,福建龙岩364012 [3]龙岩市新罗区农业局山麻鸭原种场,福建龙岩364031 [4]福建省畜牧总站,福州350003 [5]福建农林大学动物科学学院(蜂学学院),福州350002

出　　处：《中国农业科学》2023年第3期572-586,共15页Scientia Agricultura Sinica

基　　金：福建省种业创新与产业化工程(2021-2025);农业良种重大科研育种攻关与产业化工程项目(zycxny20211014);福建省科技厅对外合作项目(2021I0045);福建省科技厅引导性科技项目(2020N0034);龙岩学院科研博士启动基金(LB2019001)。

摘　　要：【目的】通过全基因组关联研究(genome-wide association study,GWAS)技术筛选和鉴定鸭蛋品质性状的单核苷酸多态性(single nucleotide polymorphisms,SNPs)位点及候选基因,为龙岩山麻鸭蛋品质性状分子育种提供参考。【方法】试验测定产蛋后期235只龙岩山麻鸭母鸭蛋品质性状,包括蛋重(egg weight,EW)、蛋形指数(egg shaped index,ESI)、蛋壳厚(eggshell thickness,EST)、蛋壳强度(eggshell strength,ESS)、蛋壳颜色L^(*)、a^(*)、b^(*)值(eggshell colour L^(*),a^(*),b^(*),ESCL、ESCA和ESCB)、蛋白高度(albumin height,AH)、哈氏单位(Haugh unit,HU)、蛋黄颜色(egg yolk colour,EYC)、蛋黄重(egg yolk weight,EYW)和蛋黄比例(egg yolk percentage relative to egg weight,EYP)。使用ASReml-R 4.1软件多性状动物模型对蛋品质性状进行遗传参数估计。使用简化基因组测序技术对鸭血液基因组DNA进行SNP分型,分型后进行蛋品质性状与这些SNPs间的GWAS研究。【结果】龙岩山麻鸭蛋品质性状中,EW、ESI、EST、ESL、ESA和AU具有中高等的遗传力,遗传力在0.21—0.70之间。EW与AU存在较强的正遗传相关(rg=0.91±0.37)。ESI与EYC存在较强的遗传负相关(rg=-0.98±1.03)。EST与ESS具有表型正相关(rp=0.41±0.06),与ESA具有遗传和表型负相关(rg=-0.86±0.25和rp=-0.15±0.07),与ESB具有遗传和表型正相关(rg=0.96±0.37和rp=0.18±0.07)。ESA与ESB具有遗传和表型负相关(rg=-0.64±0.28和rp=-0.31±0.06)。GWAS研究结果表明,7个SNPs位点与ESI、EST和EYC达到5%基因组水平显著关联(P<4.74×10^(-6)),涉及6个候选基因。与ESI关联的SNP(chr20:11135563:G:C)位点位于20号染色体含有75A富含亮氨酸重复序列(leucine rich repeat containing 75A)基因内。与EST关联的2个SNPs(chr13:5766560:A:G和chrZ:968819:C:T)位点分别位于13号LOC106014427下游6.86 kb处和Z染色体转录因子4(transcription factor 4)基因内。与EYC关联的4个SNPs位点,其中1个(chr2:38155965:G:A)位于2号染色体钾电压�【Objective】Single nucleotide polymorphisms(SNPs)and candidate genes for egg quality traits in duck were identified through genome-wide association study(GWAS)technology,so as to provide reference for molecular breeding to improve egg quality traits of Longyan Shan-Ma Duck.【Method】Egg quality traits of 235 female Longyan Shan-Ma Duck were measured,including egg weight(EW),egg shaped index(ESI),eggshell thickness(EST),eggshell strength(ESS),eggshell colour L^(*)(ESCL),a^(*)(ESCA),b^(*)(ESCB),albumin height(AH),Haugh unit(HU),egg yolk colour(EYC),egg yolk weight(EYW),and egg yolk percentage relative to egg weight(EYP).Genetic parameters of these traits were estimated using multi-trait animal model by ASReml-R 4.1 software.Blood genomic DNAs of these ducks were genotyped using genotyping-by-sequencing(GBS)technology.The GWAS between egg quality traits of the late laying period and SNPs were performed.【Result】The heritability of EW,ESI,EST,ESL,ESA and AU was higher among the egg quality traits of Longyan Shan-Ma Duck,and ranged from 0.21 to 0.70.There was a strong positive genetic correlation(rg=0.91±0.37)between EW and AU,a strong negative genetic correlation(rg=-0.98±1.03)between ESI and EYC.EST had a positive phenotypic correlation(rp=0.41±0.06)with ESS,negative genetic and phenotypic correlations with ESA(rg=-0.86±0.25 and rp=-0.15±0.07),and positive genetic and phenotypic correlations with ESB(rg=0.96±0.37 and 0.18±0.07).There were negative genetic and phenotypic correlations between ESA and ESB(rg=-0.64±0.28 and rp=-0.31±0.06).Results from the GWAS showed that seven SNPs were significantly associated with ESI,EST and yolk color(YC)at 5%Bonferroni-corrected genome-wide significance level(P<4.74×10^(-6)),involving six candidate genes.One SNP,chr20:11135563:G:C,was associated with ESI,which was in leucine rich repeat containing 75A gene,located on chromosome 20.Two SNPs,chr13:5766560:A:G and chrZ:968819:C:T,were associated with EST,which were located on chromosome 13,downstream 6.86 Kb of LOC1

关 键 词：龙岩山麻鸭 蛋品质 遗传参数 单核苷酸多态性 全基因组关联研究 

分 类 号：S834[农业科学—畜牧学]