弱畸精子症患者精子中GLUT8的表达与精子活力和形态参数关系的研究  

Study on the relationship between the expression of GLUT8 and sperm motility and morphology in asthenozoospermia and teratozoospermia patients

作　　者：郭润发 李雪芳 朱文凯 张立新[1] 葛红山 Guo Runfa;Li Xuefang;Zhu Wenkai;Zhang Lixin;Ge Hongshan(Department of Reproductive Medicine,the Affiliated Taizhou People's Hospital of Nanjing Medical University,Taizhou 225300,China;Medical Genetics and Prenatal Diagnosis Centre,the Affiliated Taizhou People's Hospital of Nanjing Medical University,Taizhou 225300,China)

机构地区：[1]南京医科大学附属泰州人民医院生殖医学科,泰州225300 [2]南京医科大学附属泰州人民医院医学遗传与产前诊断中心,泰州225300

出　　处：《中华生殖与避孕杂志》2022年第12期1259-1264,共6页Chinese Journal of Reproduction and Contraception

基　　金：国家自然科学基金(81771586);南京医科大学附属泰州人民医院院级科研基金项目(ZL201813)。

摘　　要：目的研究弱精子症(asthenospermia,AS)和畸形精子症(teratozoospermia,TM)患者精子与正常生育男性精子中葡萄糖转运蛋白8(glucose transporter 8,GLUT8)蛋白表达量的变化,探讨精子GLUT8的表达与精子活力和畸形率的相关性。方法采用计算机辅助精液分析系统(computer-assisted semen analysis system,CASA)检测各组精子运动参数。采用病例对照研究法,收集2019年12月至2020年12月期间于南京医科大学附属泰州市人民医院生殖医学科男科实验室进行精液常规检查的AS患者、TM患者和正常生育男性的精液样本。按照精子活力,分为正常活力精子(normal viable spermatozoa,NV)组与AS组,每组各41例;按照精子形态,分为正常形态精子(normal morphology spermatozoa,NM)组与TM组,每组各40例。Western blotting法检测NV组、AS组、NM组和TM组的GLUT8蛋白表达量,每组各7例。比较NV组和AS组、NM组和TM组的精子参数及GLUT8蛋白的差异。结果NV组与AS组的精子参数在活力(62.64%±37.14%、14.41%±11.89%)、活动率(55.62%±12.31%、24.40%±12.75%)、曲线速度[(87.22±12.35)µm/s、(71.75±18.35)µm/s]、直线速度[(40.22±6.71)µm/s、(33.31±10.11)µm/s)]、平均路径速度[(47.66±6.49)µm/s、(39.42±10.25)µm/s]、精子头部侧向摆动幅度[(2.88±0.49)μm、(2.35±0.66)μm]差异均有统计学意义(均P<0.001)。NM组与TM组的精子参数比较差异均无统计学意义(均P>0.05)。AS组中GLUT8蛋白表达量(0.26±0.17)低于NV组(2.00±0.38,P=0.002),而NM组与TM组GLUT8蛋白表达量差异无统计学意义(P>0.05)。结论AS患者精子中GLUT8蛋白表达量下降,而GLUT8蛋白的表达量与精子形态之间并无显著联系。Objective To study the difference of facilitative glucose transporter 8(GLUT8)expression in sperms among asthenozoospermia(AS)patients,teratozoospermia(TM)patients and normal reproductive men,thus to investigate the correlation between the expression of GLUT8 and sperm motility.Methods The sperm motility parameters of each group were detected by computer-assisted semen analysis system(CASA).A case-control study method was used to collect semen from patients with AS,TM and normal reproductive men who underwent routine semen examination in the Andrology Laboratory,Department of Reproductive Medicine,the Affiliated Taizhou People's Hospital of Nanjing Medical University from December 2019 to December 2020.The semen of AS patients(41 samples),normal viable spermatozoa(NV)patients(41 samples),TM patients(40 samples)and normal morphology spermatozoa(NM)patients(40 samples)were collected and selected into AS group,NV group,TM group and NM group according to the criteria of enrollment.Western blotting was used to assess the expression of GLUT8 protein in NV group,AS group,NM group and TM group,with 7 cases in each group.The differences of sperm parameters and GLUT8 protein between NV group and AS group,NM group and TM group were compared.Results Sperm parameters of NV group and AS group were significantly different in progressive rate(62.64%±37.14%,14.41%±11.89%,P<0.001),motility rate(55.62%±12.31%,24.40%±12.75%,P<0.001),curvilinear velocity[(87.22±12.35)µm/s,(71.75±18.35)µm/s,P<0.001],the straight line velocity[(40.22±6.71)µm/s,(33.31±10.11)µm/s,P<0.001],average path velocity[(47.66±6.49)µm/s,(39.42±10.25)µm/s,P<0.001],and amplitude of lateral head displacement[(2.88±0.49)μm,(2.35±0.66)μm,P<0.001],and there were no significant differences in sperm parameters between NM group and TM group(all P>0.05).The relative expression of GLUT8 protein in AS group(0.26±0.17)was significantly lower than that in NV group(2.00±0.38,P=0.002),but there was no significant difference in GLUT8 protein expression in NM

关 键 词：精子活力 弱精子症 葡萄糖转运蛋白8 

分 类 号：R698.2[医药卫生—泌尿科学]