氮添加诱导的磷限制改变了亚热带黄山松林土壤微生物群落结构  

Phosphorus limitation induced by nitrogen addition changed soil microbial community structure in a subtropical Pinus taiwanensis forest

作　　者：张晓晴 曾泉鑫 元晓春 万晓华[1,2] 崔琚琰 李文周 林惠瑛 谢欢 陈文伟 吴君梅 陈岳民 ZHANG Xiao-qing;ZENG Quan-xin;YUAN Xiao-cun;WAN Xiao-hua;CUI Ju-yan;LI Wen-zhou;LIN Hui-ying;XIE Huan;CHEN Wen-wei;WU Jun-mei;CHEN Yueh-min(Cultivation Base of State Key Laboratory of Humid Subtropical Mountain Ecology,Fujian Normal University,Fuzhou 350007,China;School of Geographical Science,Fujian Normal University,Fuzhou 350007,China;College of Tourism,Wuyi University,Wuyishan 354300,Fujian,China;Daiyun Mountain National Nature Reserve Administration Bureau,Quanzhou 362500,Fujian,China)

机构地区：[1]福建师范大学湿润亚热带山地生态国家重点实验室培育基地,福州350007 [2]福建师范大学地理科学学院,福州350007 [3]武夷学院旅游学院,福建武夷山354300 [4]福建戴云山国家级自然保护区管理局,福建泉州362500

出　　处：《应用生态学报》2023年第1期203-212,共10页Chinese Journal of Applied Ecology

基　　金：福建省自然科学基金项目(2020J01142,2020J01397);安徽省自然科学基金项目(2108085QC105)资助。

摘　　要：土壤微生物在陆地生态系统的生物地球化学循环中起着重要作用。然而目前尚不清楚氮(N)添加量及其持续时间如何影响土壤微生物群落结构,以及微生物群落结构变化与微生物相对养分限制状况是否存在关联。本研究在亚热带黄山松林开展了N添加试验以模拟N沉降,并设置3个处理:对照(CK,0 kg N·hm^(-2)·a^(-1))、低N(LN,40 kg N·hm^(-2)·a^(-1))和高N(HN,80 kg N·hm^(-2)·a^(-1))。在N添加满1年和3年时测定土壤基本理化性质、磷脂脂肪酸含量和碳(C)、N、磷(P)获取酶活性,并通过生态酶化学计量分析土壤微生物的相对养分限制状况。结果表明:1年N添加对土壤微生物群落结构无显著影响,3年LN处理显著提高了革兰氏阳性菌(G^(+))、革兰氏阴性菌(G^(-))、放线菌(ACT)和总磷脂脂肪酸(TPLFA)含量,而3年HN处理对微生物的影响不显著,表明细菌和ACT对N添加可能更为敏感。N添加加剧了微生物C和P限制,而P限制是土壤微生物群落结构变化的最佳解释因子。这表明,N添加诱导的P限制可能更有利于部分贫营养菌(如G^(+))和参与P循环的微生物(如ACT)的生长,从而改变亚热带黄山松林土壤微生物群落结构。Soil microorganisms play an important role in the biogeochemical cycles of terrestrial ecosystems.How-ever,it is still unclear how the amount and duration of nitrogen(N)addition affect soil microbial community structure and whether there is a correlation between the changes in microbial community structure and their nutrient limi-tation status.In this study,we conducted an N addition experiment in a subtropical Pinus taiwanensis forest to simulate N deposition with three treatments:control(CK,0 kg N·hm^(-2)·a^(-1)),low N(LN,40 kg N·hm^(-2)·a^(-1)),and high N(HN,80 kg N·hm^(-2)·a^(-1)).Basic soil physicochemical properties,phospholipid fatty acids content,and carbon(C),N and phosphorus(P)acquisition enzyme activities were measured after one and three years of N addition.The relative nutrient limitation status of soil microorganisms was analyzed using ecological enzyme stoichiometry.The results showed that one-year N addition did not affect soil microbial community structure.Three-year LN treatment significantly increased the contents of Gram-positive bacteria(G^(+)),Gram-negative bacteria(G^(-)),actinomycetes(ACT),and total phospholipid fatty acids(TPLFA),whereas three-year HN treatment did not significantly affect soil microbial community,indicating that bacteria and ACT might be more sensitive to N addition.Nitrogen addition exacerbated soil C and P limitation.Phosphorus limitation was the optimal explanatory factor for the changes in soil microbial community structure.It suggested that P limitation induced by N addition might be more beneficial for the growth of certain oligotrophic bacteria(e.g.G^(+))and the microorganisms participating in the P cycling(e.g.ACT),with consequences on soil microbial community structure of subtropical Pinus taiwanensis forest.

关 键 词：氮添加 磷酸脂肪酸 磷限制 碳限制 

分 类 号：S714.3[农业科学—林学]