芳樟醇对灰葡萄孢生长的影响及对番茄灰霉病的防控效果  

Effects of linalool on Botrytis cinerea growth and control of tomato gray mold

作　　者：王启方 王晓云[1] 李浩森 杨晓玉[1] 张锐敏 巩彪[1] 李秀明 史庆华[1] WANG Qi-fang;WANG Xiao-yun;LI Hao-sen;YANG Xiao-yu;ZHANG Rui-min;GONG Biao;LI Xiu-ming;SHI Qing-hua(College of Hor-ticulture Science and Engineering/State Key Laboratory of Crop Biology/Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient Production in Shandong,Shandong Agricultural University,Tai’an 271018,Shandong,China)

机构地区：[1]山东农业大学园艺科学与工程学院/作物生物学国家重点实验室/山东果蔬优质高效生产协同创新中心,山东泰安271018

出　　处：《应用生态学报》2023年第1期213-220,共8页Chinese Journal of Applied Ecology

基　　金：山东省蔬菜产业技术体系项目(SDAIT-05);山东省农业重大应用技术创新项目(2018);鲁渝科技协作计划项目(2020LYXZ001)资助。

摘　　要：通过平板抑菌试验和孢子萌发试验研究了芳樟醇对灰葡萄孢的生长抑制作用,并通过盆栽试验进一步验证了芳樟醇对番茄灰霉病的防控效果。结果表明:芳樟醇能够显著抑制灰葡萄孢菌丝的生长,半最大效应浓度(EC_(50))值为0.581 mL·L^(-1)。孢子萌发试验中,芳樟醇能够有效抑制灰葡萄孢孢子的萌发,并表现出浓度依赖性。芳樟醇处理提高了灰葡萄孢菌菌丝体的相对电导率和丙二醛(MDA)含量,说明芳樟醇可引起氧化损伤效应导致灰葡萄孢菌的膜系统被破坏;芳樟醇处理后灰葡萄孢菌中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性较对照组分别下降了27.4%、68.9%和26.0%,说明芳樟醇抑制了灰葡萄孢菌体内的抗氧化系统。盆栽试验结果显示,芳樟醇处理的病斑直径较对照组显著降低;番茄叶片中的SOD、CAT、POD、多酚氧化酶和苯丙氨酸解氨酶活性均显著高于对照组;而MDA含量下降了41.5%,说明芳樟醇可减轻灰葡萄孢菌对番茄植株造成的氧化损伤以提高植物抗病性。综上,芳樟醇对灰葡萄孢的生长有显著抑制作用并对番茄灰霉病有较好的防治效果,研究结果可为开发新型植物源抑菌剂防控番茄灰霉病提供理论依据。We examined the antifungal characteristics of linalool against Botrytis cinerea using plate inhibition assay and spore germination assay,and assessed the capacity of linalool in controlling tomato gray mold disease via tomato pot inoculation assay.The results showed that linalool exhibited strong inhibitive effects on mycelial growth of B.cinerea,with an EC_(50) value of 0.581 mL·L^(-1).In the spore germination test,linalool treatment inhibited spore germination in a dose-dependent manner.The electric conductivity and the malondialdehyde(MDA)contents were significantly increased in linalool-treated B.cinerea than that of the control,indicating that linalool induced oxidative damage and destroyed the cell membrane integrity in B.cinerea.The activities of the superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD)in the linalool-treated B.cinerea were decreased significantly by 27.4%,68.9%and 26.0%,respectively,suggesting that linalool inhibited the antioxidant activity of B.cinerea.In the pot experiment,the diameter of lesions in linalool-treated tomatoes was significantly smaller than that of the control.The activities of SOD,POD,CAT,polyphenol oxidase,and phenylalnine ammonialyase in the linalool-treated tomatoes increased,while the MDA content decreased,suggesting that linalool could alleviate the oxidative damage caused by B.cinerea and promote plant disease resistance.In summary,linalool had inhibitory effect on the growth of B.cinerea and could control gray mold disease in tomatoes.These findings could lay the foundation for developing bota-nical antifungal agents for management of tomato gray mold disease.

关 键 词：灰葡萄孢 芳樟醇 番茄 灰霉病 

分 类 号：S436.412.13[农业科学—农业昆虫与害虫防治]