马勃油膏对兔大肠杆菌感染性创面NLRP3/Caspase-1炎症通路及炎症因子表达的影响  

Effect of Mabo Ointment(马勃油膏)on NLRP3/Caspase-1 Inflammatory Pathway and Expression of Inflammatory Factors in Rabbits with Escherichia Coli Infectious Wound

作　　者：杨爱龙[1,2] 韩雪[1,2] 于波[1,3] 阚成国[1,2] 喻少雷 YANG Ailong;HAN Xue;YU Bo;KAN Chengguo;YU Shaolei(Heilongjiang University of Chinese Medicine,Harbin Heilongjiang 150000,China;The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin Heilongjiang 150000,China;The Fourth Affiliated Hospital of Harbin Medical University,Harbin Heilongjiang 150000,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150000 [2]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150000 [3]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150000

出　　处：《中医药导报》2023年第1期24-30,共7页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：黑龙江中医药大学科研基金项目(201729);黑龙江省自然科学基金项目(H2017069)。

摘　　要：目的:基于NLRP3/Caspase-1炎症通路探讨马勃油膏对兔大肠杆菌感染性创面愈合情况及炎症因子表达的影响。方法:将24只新西兰大白兔随机分为空白对照组、模型组、马勃油膏组和紫草生肌搽剂组,每组6只。模型组、马勃油膏组和紫草生肌搽剂组兔制备大肠杆菌感染性创面模型,造模成功后马勃油膏组和紫草生肌搽剂组分别予马勃油膏、紫草生肌搽剂外敷,空白对照组和模型组予生理盐水清洗创面,在干预第3、7、14天,分别观察创面愈合情况,测定创面愈合率,HE染色观察创面组织病理变化,ELISA检测创面组织中IL-1β、TNF-α含量,Western blotting检测创面组织中NLRP3、Caspase-1蛋白法表达情况。结果:空白对照组兔创面结痂、愈合的速度最快;模型组兔创面红肿明显,愈合的速度最慢,渗血、渗液较多,后期可见明显化脓;马勃油膏组和紫草生肌搽剂组兔创面渗血、渗液较少,未见化脓,肉芽组织生长旺盛,创面结痂、愈合的速度较快。HE染色显示,空白对照组兔创面较少见炎症细胞浸润,模型组兔创面的炎症细胞浸润程度逐渐严重,而马勃油膏组和紫草生肌搽剂组兔创面的炎症细胞浸润程度逐渐减轻。干预第3、7、14天,马勃油膏组兔的创面愈合率明显高于模型组(P<0.05);马勃油膏组兔创面组织中的IL-1β、TNF-α含量及NLRP3、Caspase-1蛋白表达均明显低于模型组(P<0.05);马勃油膏组兔的创面愈合率、创面组织中的IL-1β、TNF-α含量及创面组织中NLRP3、Caspase-1表达与紫草生肌搽剂组比较,差异均无统计学意义(P>0.05)。结论:马勃油膏可通过干预NLRP3/Caspase-1炎症通路降低兔大肠杆菌感染性创面的炎症因子表达,加速创面愈合,其疗用与紫草生肌搽剂相当。Objective:Based on the NLRP3/Caspase-1 inflammatory pathway,to explore the effect of Mabo Ointment on the healing of rabbit infected wounds caused by Escherichia coli and the expression of inflammatory factors.Methods:24 New Zealand white rabbits were randomly divided into blank control group,model group,Mabo Ointment group and Zicao Shengji Liniment(紫草生肌搽剂)group,with 6 rabbits in each group.The rabbits in the model group,the Mabo Ointment group and the Zicao Shengji Liniment group were used to prepare the wound model of Escherichia coli infection.After the successful model establishment,the Mabo Ointment group and the Zicao Shengji Liniment group were applied externally with Mabo Ointment and Zicao Shengji Liniment,respectively,and the blank control group and model group were given saline to wash the wound.On the 3rd,7th and 14th day of the intervention,the wound healing was observed,the wound healing rate was measured,the pathological changes of the wound tissue were observed by HE staining,the content of IL-1βand TNF-αin the wound tissue was detected by ELISA,and the expression of NLRP3 and Caspase-1 protein in the wound tissue was detected by Western blotting.Results:The wound surface of rabbits in the blank control group had the fastest rate of scab formation and healing.The rabbits in the model group had obvious redness and swelling on the wound surface,the healing speed was the slowest,there was more blood and fluid seepage,and obvious suppuration could be seen in the later stage.The rabbits in the Mabo Ointment group and the Zicao Shengji Liniment group had less blood and fluid oozing from the wound surface,no suppuration,the granulation tissue grew vigorously,and the wounds were scabbed and healed faster.HE staining showed that there was less inflammatory cell infiltration in the wounds of the blank control group.The degree of inflammatory cell infiltration in the wounds of the model group was gradually serious,while the degree of inflammatory cell infiltration in the wounds of the Mabo Oin

关 键 词：大肠杆菌感染性创面 马勃油膏 NLRP3/Caspase-1炎症通路 白介素-6 肿瘤坏死因子-α 创面修复 紫草生肌搽剂 兔 

分 类 号：R285.5[医药卫生—中药学]