miR-485-3p过表达对P19细胞增殖、凋亡及分化的影响  

Effect of miR-485-3p overexpression on the proliferation,apoptosis and differentiation of P19 cells

作　　者：沙静[1] 黄桂平 徐早春 郭梨梨 翟敬芳[1] Sha Jing;Huang Guiping;Xu Zaochun;Guo Lili;Zhai Jingfang(Prenatal Diagnosis Center,Xuzhou Central Hospital,Xuzhou 221009,Jiangsu,China;Physical Examination Center,Xuzhou Central Hospital,Xuzhou 221009,Jiangsu,China;Bengbu Medical College,Bengbu 233000,Anhui,China)

机构地区：[1]徐州市中心医院产前诊断中心,江苏徐州221009 [2]徐州市中心医院体检中心,江苏徐州221009 [3]蚌埠医学院,安徽蚌埠233000

出　　处：《中国产前诊断杂志（电子版）》2022年第4期41-45,61,共6页Chinese Journal of Prenatal Diagnosis(Electronic Version)

基　　金：徐州市科学技术局重点研发计划项目(KC19153、KC21189)。

摘　　要：目的探讨miR-485-3p过表达对P19细胞增殖、凋亡及分化的影响。方法体外传代培养P19细胞,利用细胞转染技术构建miR-485-3p过表达细胞。CCK-8试剂盒检测细胞增殖;流式细胞技术检测细胞凋亡情况;P19细胞在体外二甲基亚砜(DMSO)诱导下向心肌细胞分化,应用实时荧光定量PCR和Western blot检测诱导分化第0、4、8、10d心肌分化标志物心肌肌钙蛋白T(cTnT)、GATA结合蛋白4(GATA4)和心肌增强因子2c(Mef2c)的表达情况,明确miR-485-3p过表达对P19细胞分化的影响。结果与对照组相比,miR-485-3p过表达组细胞增殖速率较慢,细胞凋亡率增高,心肌细胞分化过程中的cTnT、GATA4和Mef2c三个标志基因均显著下调。结论miR-485-3p过表达能够抑制P19细胞增殖、促进其凋亡并抑制其向心肌细胞的分化作用,本研究为先天性心脏病的防治提供了理论依据。Objective To explore the effect of miR-485-3p overexpression on P19 cell proliferation,apoptosis and differentiation.Methods P19 cells were cultured and transfected with miR-485-3p mimics.CCK-8 assay was performed to assess theproliferation,flow cytometry was used to analyse the apoptosis.P19 cells were induced to differentiate with DMSO.RT-qPCR and Western blotting wereusedtodetectthe cardiomyogenesis related RNAs and proteins expressionof cTnT,GATA4,and Mef2cat day 0,4,8,and day 10.Results Thedatashowed that thecellproliferationrate of miR-485-3p overexpression group was decreased compared with the control group,the cell apoptosis rate was increased.miR-485-3p overexpression obviously down-regulated the expression ofcardiomyogenic-specific molecular markers(cTnT,GATA4,and Mef2c).Conclusion Overexpression of miR-485-3p can inhibits proliferation and differentiation and promotes apoptosis in P19cells,thus providing a potentialtherapeutic evidence for congenital heart disease.

关 键 词：miR-485-3p 先天性心脏病 增殖 凋亡 分化 

分 类 号：R715.5[医药卫生—妇产科学]