Application and development of fluorescence probes in MINFLUX nanoscopy(invited paper)  

作　　者：Jing Wang Zhen Zhang Hongyu Shen Qi Wu Min Gu 

机构地区：[1]Institute of Photonic Chips University of Shanghai for Science and Technology Shanghai 200093,P.R.China [2]Centre for Articial-Intelligence Nanophotonics School of Optical-Electrical and Computer Engineering University of Shanghai for Science and Technology Shanghai 200093,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2023年第1期67-78,共12页创新光学健康科学杂志（英文）

基　　金：supported by the Science and Technology Commission of Shanghai Municipality (21DZ1100500);the Shanghai Municipal Science and Technology Major Project;the Shanghai Frontiers Science Center Program (2021-2025 No.20);Shanghai Hong Kong,Macao,and Taiwan Cooperation Project (No.19490760900).

摘　　要：The MINimal emission FLUXes(MINFLUX)technique in optical microscopy,widely recognized as the next innovative fluorescence microscopy method,claims a spatial resolution of 1-3 nm in both dead and living cells.To make use of the full resolution of the MINFLUX microscope,it is important to select appropriate fluorescence probes and labeling strategies,especially in living-cell imaging.This paper mainly focuses on recent applications and developments of fluorescence probes and the relevant labeling strategy for MINFLUX microscopy.Moreover,we discuss the deficiencies that need to be addressed in the future and a plan for the possible progression of MINFLUX to help investigators who have been involved in or are just starting in the field of super-resolution imaging microscopy with theoretical support.

关 键 词：Fluorescence probes MINFLUX nanoscopy photoblinking super-resolution imaging labeling strategy 

分 类 号：O657.3[理学—分析化学]