辽宁省1~30岁健康人群水痘-带状疱疹病毒IgG抗体血清学分析  

Serological analysis of varicella-zoster virus IgG antibody in healthy people aged 1~30 years in Liaoning Province

作　　者：王文思[1] 方兴[1] 任丽萍[1] 安晓慧[1] 韩悦[1] 王艳[1] WANG Wen-si;FANG Xing;REN Li-ping;AN Xiao-hui;HAN Yue;WANG Yan(Liaoning Province Center for Disease Control and Prevention,Shenyang 110005,Liaoning Province,China)

机构地区：[1]辽宁省疾病预防控制中心,辽宁沈阳110005

出　　处：《中国生物制品学杂志》2023年第1期70-75,共6页Chinese Journal of Biologicals

基　　金：辽宁省科学技术计划项目(2020-MS-357)。

摘　　要：目的对辽宁省1~30岁健康人群水痘-带状疱疹病毒(varicella-zoster virus,VZV)IgG抗体水平进行血清学分析。方法于2020年10月采用分层随机抽样方法抽取辽宁省沈阳市、阜新市及丹东市3个地区的6个县区作为监测点,采集617名1~30岁健康人群的静脉血3~5 mL,分离血清,ELISA法检测VZV IgG抗体水平,计算血清抗体阳性率和抗体几何平均浓度(geometric mean concentration,GMC),并进行比较分析。结果617份血清样本中共检出VZV IgG抗体阳性样本302份,阳性率为48.947%,GMC为112.772 mIU/mL。不同年龄组健康人群VZV IgG抗体阳性率为29.670%~75.789%,GMC为45.508~366.559 mIU/mL,抗体阳性率(χ^(2)=67.104,P<0.001)和GMC(F=20.685,P<0.001)差异均有统计学意义。男性和女性的VZV IgG抗体阳性率分别为44.817%和53.633%,差异有统计学意义(χ^(2)=4.779,P=0.029);GMC分别为96.983和133.829 mIU/mL,差异无统计学意义(t=-1.958,P=0.051)。辽宁省沈阳市、阜新市、丹东市健康人群VZV IgG抗体阳性率分别为55.224%、40.201%、51.152%,3个地区抗体阳性率差异有统计学意义(χ^(2)=9.683,P=0.008),阜新市明显低于沈阳市及丹东市(χ^(2)分别为9.046和5.013,P分别为0.003和0.025);GMC分别为133.523、85.953、123.713 mIU/mL,3个地区GMC差异无统计学意义(F=0.514,P=0.598)。617份血清样本中,54份为可疑结果,复检后仍在临界范围内,随年龄增长阳性率与阳性和可疑结果合计百分率之间的差距逐渐减小,表明随着年龄的增长对水痘的免疫力逐渐增强。结论辽宁省1~30岁健康人群VZV IgG抗体水平随年龄增长逐渐升高,但总体水平偏低。为控制水痘病毒的传播,建议提高薄弱地区和易感人群的水痘疫苗覆盖率,以建立VZV免疫屏障。Objective To carry out serological analysis of varicella-zoster virus(VZV)IgG antibody level in healthy people aged 1~30 years in Liaoning Province.Methods In October 2020,3~5 mL venous blood samples were collected from 617 healthy people aged 1~30 years selected from six counties and districts in Shenyang,Fuxin and Dandong of Liaoning Province by stratified random sampling method,of which serum samples were collected and determined for VZV IgG antibody level by ELISA.The positive rate of serum antibody and geometric mean concentration(GMC)of antibody were calculated and compared.Results Among 617 serum samples,302 samples were positive for VZV IgG antibody,the positive rate was48.947%,and the GMC was 112.772 mIU/mL.The positive rate of VZV IgG antibody was 29.670%~75.789%and the GMC was 45.508~366.559 mIU/mL in healthy people of various ages.Both of the antibody positive rate(χ^(2)=67.104,P<0.001)and GMC(F=20.685,P<0.001)showed significant differences.The positive rates of VZV IgG antibody in male and female were 44.817%and 53.633%respectively,which showed significant difference(χ^(2)=4.779,P=0.029),while the GMCs were 96.983 and 133.829 mIU/mL respectively(t=-1.958,P=0.051)with no significant difference.The positive rates of VZV IgG antibody of healthy people in Shenyang,Fuxin and Dandong of Liaoning Province were 55.224%,40.201%and 51.152%respectively with significant differences(χ^(2)=9.683,P=0.008),of which the positive rate of Fuxin was significantly lower than those of Shenyang and Dandong(χ^(2)=9.046 and 5.013,P=0.003 and 0.025,respectively);While the GMCs were 133.523,85.953 and 123.713 mIU/mL respectively with no significant difference(F=0.514,P=0.598).Among 617 serum samples,54 samples were suspicious,which remained within the critical range after re-examination,while the gap between positive rate and the total percentage of positive and suspicious results gradually decreased with the increase of age,indicating that the immunity to varicella gradually increased with the increase of age.Conclusion T

关 键 词：水痘-带状疱疹病毒 IGG抗体 抗体阳性率 血清学分析 

分 类 号：R511.5[医药卫生—内科学]