大黄素对肺炎链球菌诱导的肺泡Ⅱ型上皮细胞凋亡和炎症的影响  

Effect of emodin on apoptosis and inflammation of alveolar type Ⅱ epithelial cells induced by Streptococcus pneumoniae

作　　者：白蔷薇 李翠凡 李梦媛 任静婕 崔珍逖 BAI Qiang-wei;LI Cui-fan;LI Meng-yuan;REN Jing-jie;CUI Zhen-ti(Teaching and Research Section,Zhengzho Su ias College,Zhengzhou450000,Henan Province,China;Basic Medicine Teaching and Research Section,Zhengzho Su ias College,Zhengzhou450000,Henan Province,China)

机构地区：[1]郑州西亚斯学院教研室,河南郑州450000 [2]郑州西亚斯学院基础医学教研室,河南郑州450000

出　　处：《中国临床药理学杂志》2022年第24期2959-2963,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的 探究大黄素对肺炎链球菌诱导的肺泡Ⅱ型上皮细胞凋亡和炎症影响及机制。方法 人肺泡上皮细胞HPAEpiC细胞分成对照组、模型组(肺炎链球菌处理)、实验低剂量组(肺炎链球菌和5μg·mL^(-1)大黄素处理)、实验中剂量组(肺炎链球菌和10μg·mL^(-1)大黄素处理)、实验高剂量组(肺炎链球菌和15μg·mL^(-1)大黄素处理)、实验高剂量+vector组(转染阴性对照载体,肺炎链球菌和15μg·mL^(-1)大黄素处理)、实验高剂量+基质金属蛋白酶组织抑制因子1(TIMP-1)组(转染TIMP-1过表达载体,肺炎链球菌和15μg·mL^(-1)大黄素处理)。以噻唑蓝(MTT)法测定细胞增殖,用流式细胞术检测细胞凋亡,用酶联免疫吸附(ELISA)法测定培养液上清中白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)含量,以蛋白质印迹法检测活化的胱天蛋白酶-3(C-caspase-3)蛋白表达。结果 对照组、模型组、实验低剂量组、实验中剂量组、实验高剂量组、实验高剂量+vector组、实验高剂量+TIMP-1组细胞增殖活力(OD值)分别为0.73±0.04,0.37±0.03,0.41±0.03,0.54±0.05,0.64±0.03,0.65±0.05和0.46±0.04;凋亡率分别为(7.66±0.81)%,(27.23±3.02)%,(18.84±1.70)%,(15.89±0.72)%,(12.26±0.74)%,(13.20±0.92)%和(15.83±1.58)%;C-caspase-3蛋白表达水平分别为0.38±0.03,0.86±0.10,0.68±0.10,0.53±0.05,0.43±0.04,0.42±0.03和0.64±0.05;培养液上清中IL-1β含量分别为(10.88±1.14),(17.65±0.91),(15.79±1.55),(13.23±1.16),(11.43±0.75),(11.78±1.51)和(15.57±0.48)ng·L^(-1);TNF-α含量分别为(1.36±0.15),(2.14±0.20),(1.87±0.13),(1.66±0.14),(1.50±0.12),(1.45±0.07)和(1.82±0.22)ng·L^(-1);IL-8含量分别为(0.32±0.03),(1.07±0.06),(0.85±0.07),(0.63±0.05),(0.53±0.04),(0.51±0.07)和(0.76±0.09)ng·L^(-1)。以上指标,模型组与对照组比较,差异均有统计学意义(均P<0.05);实验低、中、高剂量组与模型组比较,差异均有统计学意义(均P<0.05);实验低、中剂�Objective To explore the effect and mechanism of emodin on the apoptosis and inflammation of alveolar typeⅡepithelial cells induced by Streptococcus pneumoniae.Methods Human alveolar epithelial cells HPAEpi C were divided into control group,model group(treated with Streptococcus pneumoniae),experimental low-dose group (treated with Streptococcus pneumoniae and 5μg·m L^(-1)emodin),experimental medium-dose group (treated with Streptococcus pneumoniae and 10μg·m L^(-1)emodin),experimental high-dose group (treated with Streptococcus pneumoniae and 15μg·m L^(-1)emodin),experimental high-dose+vector group (transfected negative control vector,Streptococcus pneumoniae and 15μg·m L^(-1)emodin),experimental high-dose+tissue inhibitors of MMP 1(TIMP-1) group (transfected with TIMP-1 overexpression vector,Streptococcus pneumoniae and 15μg·m L^(-1)emodin).Cell proliferation were measured by MTT method,cell apoptosis were detected by flow cytometry,interleukin-8(IL-8),tumor necrosis factor-α(TNF-α) and interleukin-1β (IL-1β) content in the culture supernatant were determined by enzyme linked immunosorbent assay (ELISA),the protein expression of Cleaved cysteinyl aspartate specific proteinase 3(C-caspase-3) were detected by Western blot.Results The cell proliferation activities (OD value) of control group,model group,experimental low-dose group,experimental medium-dose group,experimental high-dose group,experimental high-dose+vector group and experimental high-dose+TIMP-1 group were 0.73±0.04,0.37±0.03,0.41±0.03,0.54±0.05,0.64±0.03,0.65±0.05 and 0.46±0.04,respectively;the apoptosis rates were (7.66±0.81)%,(27.23±3.02)%,(18.84±1.70)%,(15.89±0.72)%,(12.26±0.74)%,(13.20±0.92)%and(15.83±1.58)%;C-caspase-3 protein expression levels were 0.38±0.03,0.86±0.10,0.68±0.10,0.53±0.05,0.43±0.04,0.42±0.03 and 0.64±0.05;IL-1β contents in the culture supernatant were (10.88±1.14),(17.65±0.91),(15.79±1.55),(13.23±1.16),(11.43±0.75),(11.78±1.51) and(15.57±0.48) ng·L^(-1);TNF-α contents were (1.36±0.

关 键 词：大黄素 肺炎链球菌 肺泡上皮细胞 基质金属蛋白酶组织抑制因子1 

分 类 号：R28[医药卫生—中药学]