Novel Deletion in Exon 7 of Betaine Aldehyde Dehydrogenase 2(BADH2)  

作　　者：VAN Quoc Giang HUYNH Ky NGUYEN Chau Thanh Tung NGUYEN Loc Hien NGUYEN van Manh NGUYEN Nhut Thanh VO Cong Thanh SWEE Keong Yeap 

机构地区：[1]College of Agriculture,Can Tho University,Can Tho 92000,Vietnam [2]Department of Sciences and Technology of Long An Province,Long An 850000,Vietnam [3]China-Association of Southeast Asian Nations College of Marine Sciences,Xiamen University Malaysia,Sepang 49000,Selangor,Malaysia

出　　处：《Rice science》2023年第2期104-112,I0020-I0030,共20页水稻科学（英文版）

基　　金：funded in part by the Can Tho University Improvement Project VN14-P6 supported by a Japanese Official Development Assistance loan。

摘　　要：The fragrance of rice is one of the premium characteristics that breeders want to include in rice varieties due to the higher market value. Nucleotide deletions in exons 2(7 bp) and 7(8 bp) of Betaine Aldehyde Dehydrogenase 2(BADH2) are associated with fragrance in rice. In this study, a new 13 bp deletion in exon 7 of the BADH2 gene was discovered in the Nang Thom Cho Dao(NTCD) variety, and the mutation has been closely related to the genetic background of indica subspecies through the Bayesian phylogenetic approach and haplotype network analysis of the 3 000 Rice Genomes Project. In addition, a set of functional markers(EX07-13F, EX07-13RN, and EX07-13RM) identified the 13 bp deletion only within NTCD(no amplified band) compared with both non-aromatic and other aromatic rice varieties(110 bp band). The deletion of 13 bases instead of 8 bases in exon 7 of BADH2 caused a premature stop codon, which down-regulated the expression of the BADH2 transcript while associated with up-regulation of OsP5CS and the high amount of 2-acetyl-1-pyrroline. It is potential to use the deletion in exon 7 of the BADH2 gene as a novel marker for adulteration and breeding of fragrant rice varieties, particularly for NTCD.

关 键 词：novel deletion BADH2 fragrant rice functional marker 

分 类 号：S511[农业科学—作物学]