银杏黄酮通过p38MAPK通路抑制口腔溃疡大鼠的炎症反应  

Ginkgo flavone inhibits the inflammatory response of oral ulcer rats through p38MAPK pathway

作　　者：张跃[1] 方超[1] 张非煜[1] ZHANG Yue;FANG Chao;ZHANG Fei-yu(Department of Stomatology,Beijing Anzhen Hospital,Capital Medical University,Beijing 100029,China)

机构地区：[1]首都医科大学附属北京安贞医院口腔科,北京100029

出　　处：《临床和实验医学杂志》2022年第23期2468-2472,共5页Journal of Clinical and Experimental Medicine

基　　金：北京市科学技术委员会项目(编号:BHT19200142)。

摘　　要：目的研究银杏黄酮通过p38促分裂原活化蛋白激酶(p38MAPK)通路抑制口腔溃疡大鼠的炎症反应。方法将56只成年雄性SD大鼠按照随机数字表法将SD大鼠分为7组,每组各8只。对照组大鼠不做任何处理,其余组采用化学烧灼法进行口腔溃疡造模。模型组采用0.9%氯化钠溶液灌胃,银杏黄酮组采用20 mg/mL银杏黄酮溶液灌胃,溶剂对照组采用DMSO腹腔注射,溶剂+模型组采用DMSO腹腔注射及0.9%氯化钠溶液灌胃,溶剂+银杏黄酮组采用DMSO腹腔注射及20 mg/mL银杏黄酮溶液灌胃,激动剂+银杏黄酮组采用p38MAPK激动剂2 mg/kg腹腔注射及20 mg/mL银杏黄酮溶液灌胃,均1次/d,连续30 d。治疗前及治疗后测量溃疡面积,检测溃疡中炎症细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6的含量以及p-p38MAPK的表达水平。结果对照组未出现口腔溃疡。治疗后10、20、30 d,银杏黄酮组大鼠的溃疡面积小于模型组,溶剂+银杏黄酮组大鼠的溃疡面积小于溶剂+模型组,激动剂+银杏黄酮组大鼠的溃疡面积大于溶剂+银杏黄酮组,差异均有统计学意义(P<0.05)。治疗后30 d,模型组溃疡组织中TNF-α、IL-1β、IL-6的含量及p-p38MAPK的表达水平均高于对照组,差异均有统计学意义(P<0.05);银杏黄酮组溃疡组织中TNF-α、IL-1β、IL-6的含量及p-p38MAPK的表达水平均低于模型组,差异均有统计学意义(P<0.05);激动剂+银杏黄酮组溃疡组织中TNF-α、IL-1β、IL-6的含量及p-p38MAPK的表达水平均高于溶剂+银杏黄酮组,差异均有统计学意义(P<0.05)。结论银杏黄酮治疗口腔溃疡大鼠显著促进溃疡愈合,抑制溃疡炎症反应,这一作用与抑制p38MAPK通路有关。Objective To study the inhibitory effect of Ginkgo flavone on inflammatory response of oral ulcer rats through p38 mitogen activated protein kinase(p38MAPK)pathway.Methods Fifty-six adult male SD rats were divided into 7 groups according to the random number table method,8 rats in each group.The rats in the control group were not received any treatment,and the rats in other groups were used to make models of oral ulcer by chemical cautery.The model group was given 0.9%sodium chloride solution by gavage,the ginkgo flavone group was given 20 mg/mL ginkgo flavone solution by gavage,the solvent control group was given DMSO intraperitoneal injection,the solvent+model group was given DMSO intraperitoneal injection and 0.9%sodium chloride solution by gavage,the solvent+ginkgo flavone group was given DMSO intraperitoneal injection and 20 mg/mL ginkgo flavone solution by gavage,the agonist+ginkgo flavone group was given p38MAPK agonist 2 mg/kg intraperitoneal injection and 20 mg/mL ginkgo flavone solution by gavage,once a day for 30 consecutive days.The ulcer area was measured before and after treatment,and the content of inflammatory cytokines tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,IL-6 and the expression level of p-p38MAPK were detected.Results There was no oral ulcer in the control group.Ten,20 and 30 days after treatment,the ulcer area of ginkgo flavone group was smaller than that of model group,and the ulcer area of rats in the solvent+ginkgo flavone group was smaller than that in the solvent+model group,and the ulcer area of rats in the agonist+ginkgo flavone group was larger than that in the solvent+ginkgo flavone group,the differences were statistically significant(P<0.05).Thirty days after treatment,the contents of TNF-α,IL-1β,IL-6 and the expression level of p-p38MAPK in ulcer tissue of model group were higher than those in the control group,the differences were statistically significant(P<0.05);the contents of TNF-α,IL-1β,IL-6 and the expression level of p-p38MAPK in ulcer tissue of ginkgo fla

关 键 词：大鼠 口腔溃疡 银杏黄酮 炎症反应 P38MAPK 

分 类 号：R285.5[医药卫生—中药学]