Electroporation of embryonic chick skin explant  

作　　者：Tailin Chen Wanjuan Bu Jun Guo Yuxiao Zhang Min Tan Jing Du Yubo Fan 

机构地区：[1]Key Laboratory of Biomechanics and Mechanobiology(Beihang University),Ministry of Education,Beijing Advanced Innovation Center for Biomedical Engineering,School of Biological Science and Medical Engineering,Beihang University,Beijing,100083,China

出　　处：《Medicine in Novel Technology and Devices》2022年第4期386-389,共4页医学中新技术与新装备（英文）

基　　金：the National Natural Science Foundation of China(12222201,82273500,11972206,11902114,U20A20390,11827803,and 11902020);Fundamental Research Funds for the Central Universities(ZG140S1971)。

摘　　要：Since the introduction of electroporation a decade ago,the chick embryo has evolved into a powerful system for studying gene regulation and function during development.Although this is a straightforward method for embryos,skin(especially lamellar tissue)present special challenges.Here we describe a protocol for electroporation of expression vectors into the chick skin explant,with precise spatial and temporal control.We have improved the design of the electroporation chamber,which can achieve precise regulation of the electroporation area by changing the parameters of the laser cutting of the acrylic plate.We give the electroporation parameters of chicken embryo skin,which can effectively improve the electroporation efficiency.This technique can be used for time-lapse imaging and to study gene regulation in chick embryo skin.

关 键 词：ELECTROPORATION Embryonic chick skin explant Electroporation chamber 

分 类 号：Q253[生物学—细胞生物学]