基于转录组测序的斧翅沙芥(Pugionium dolabratum)干旱胁迫应答基因表达分析  

Transcriptiomic analysis of drought response genes in Pugionium dolabratum

作　　者：王玮[1] 王悦[1] 厉万荣 王浩澎 杨宁[1] Wang Wei;Wang Yue;Li Wanrong;Wang Haopeng;Yang Ning(College of Life Science,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《中国沙漠》2022年第6期204-210,共7页Journal of Desert Research

基　　金：国家自然科学基金项目(32060168);甘肃省高等学校创新能力提升项目(2019B-040);西北师范大学青年教师科研能力提升计划项目(NWNU-LKQN-17-4)。

摘　　要：荒漠植物斧翅沙芥(Pugionium dolabratum)主要分布在中国西北地区的固定或半固定沙漠,具有较高的干旱环境适应能力,但对它的干旱胁迫耐受分子机制研究较少。本研究利用Illumina测序技术筛选斧翅沙芥干旱应答基因,通过转录组的从头组装,共获得154875个unigenes,平均长度为1437个碱基。干旱胁迫后,共有13855个基因表达发生显著变化,其中7182个基因上调表达,6673个基因下调表达。GO功能富集分析表明差异表达基因显著富集到物质代谢、氧化还原和催化活性等功能;KEGG通路富集分析表明差异表达基因显著富集到氨基酸代谢、淀粉和糖代谢以及植物激素信号转导等途径。此外,鉴定到47个干旱诱导转录因子家族,主要包括HSF、ERF、WRKY、MYB、bHLH等。与沙芥相比,淀粉和糖代谢途径以及大量热胁迫转录因子在干旱胁迫后上调表达有可能是斧翅沙芥提高干旱胁迫耐受能力的特殊途径。本研究的结果初步揭示了斧翅沙芥对抗干旱胁迫的调控特征,为进一步理解斧翅沙芥干旱环境适应的分子机制提供线索。Pugionium dolabratum is a xerophytic plant species mainly distributed in fixed or semi-fixed desert in northwest China,which possess strong adaptability to arid environment.However,the molecular mechanism of drought stress tolerance in P.dolabratum was known much less.In this study,we used Illumina sequencing to screen drought responsive genes in P.dolabratum,154875 unigenes with an average length of 1437 nt were obtained by De novo transcriptome assembly.After drought stress,a total of 13855 unigenes was significantly and differentially expressed,of which 7182 genes were up-regulated and 6673 genes were down regulated.GO enrichment analysis showed that the differentially expressed genes were significantly enriched in material metabolism,oxidoreductase and catalytic activity.KEGG enrichment analysis showed that the differentially expressed genes were significantly enriched in amino acid metabolism,starch and sucrose metabolism and plant hormone signal transduction.In addition,we identified 47 drought induced transcription factor families,mainly including HSF,ERF,WRKY,MYB,and bHLH.Compared with P.cornutum,the starch and sucrose metabolism and the up-regulated expression of heat stress transcription factors may be an alternative pathway to enhance tolerance capacity in P.dolabratum in response to drought stress.The results preliminarily revealed the regulatory characteristics of P.dolabratum in response to drought stress,and provided important clues for further insight into the molecular mechanism of drought environment adaptation in P.dolabratum.

关 键 词：斧翅沙芥(Pugionium dolabratum) 干旱胁迫 转录组 转录因子 

分 类 号：Q948.11[生物学—植物学]