Stimbiotic supplementation modulated intestinal inflammatory response and improved broilers performance in an experimentally-induced necrotic enteritis infection model  

作　　者：Ji Hwan Lee Byongkon Lee Xavière Rousseau Gilson A.Gomes Han Jin Oh Yong Ju Kim Se Yeon Chang Jae Woo An Young Bin Go Dong Cheol Song Hyun Ah Cho Jin Ho Cho 

机构地区：[1]Department of Animal Science,Chungbuk National University,Cheongju 28644,South Korea. [2]Cherrybro Co.,Ltd.,Jincheon‑Gun 27820,South Korea. [3]AB Vista,Marlborough,Wiltshire,UK.

出　　处：《Journal of Animal Science and Biotechnology》2023年第1期322-338,共17页畜牧与生物技术杂志（英文版）

基　　金：AB Vista(Marlborough,Wiltshire,UK)for providing the feed additives and funding this research。

摘　　要：Background:Two experiments were conducted to establish an optimal NE challenge model and evaluate the efficacy of stimbiotic(STB)supplementation in necrotic enteritis(NE)challenged broilers.In Exp.1,a total of 120 Arbor Acres(AA)broilers(45.0±0.21 g)were randomly assigned to 6 treatments in a 3×2 factorial arrangement.Vaccine treatments included non-challenge(0),×10 the recommended dose(×10)or×20 the recommended dose(×20)by the manufacturer.Clostridium perfringens(CP)treatments were non-challenge(No)or 3 mL of 2.2×10^(7)CFU CP challenge(Yes).In Exp.2,a total of 72 AA broilers(40.17±0.27 g)were randomly assigned to 6 treatments in a 3×2 factorial arrangement.Dietary treatments included non-additive(CON),100 mg/kg STB(STB)and 100 mg/kg STB on top of a typical commercial blend including an essential oil,probiotics,and enzyme(CB).Challenge treatments included non-NE challenge(No)and NE challenge(Yes)as established in Exp.1.Results:In Exp.1,CP and vaccine challenge decreased(P<0.05)body weight(BW),body weight gain(BWG)and feed intake(FI),and increased(P<0.05)the number of broilers with diarrhea and intestinal lesions.The oral administration of×20 recommended dose of vaccines coupled with 3 mL of 2.2×10^(7)CFU CP resulted in(P<0.01)a significantly increased incidence of wet litter and intestinal lesions.Thus,this treatment was chosen as the challenge model for the successful inducement of NE in Exp.2.In Exp.2,the NE challenge negatively affected(P<0.01)growth performance,ileal morphology,immunoglobulin contents in blood,caecal microbiota in the caecum,footpad dermatitis,intestinal lesion scores,tumour necrosis factor(TNF-α)and endotoxin in the serum compared with the non-NE challenged birds.The supplementation of STB and CB in diets enhanced(P<0.05)growth performance,intestinal microbiota,and blood profiles by stimulating ileal morphology(VH and VH:CD)and propionate production in the cecum,and there were no differences in measured variables between STB and CB supplemented birds.Conclusion:Overall,these resu

关 键 词：Broiler chicken Clostridium perfringens Necrotic enteritis Xylanse XYLOOLIGOSACCHARIDE 

分 类 号：S831[农业科学—畜牧学]