清肺排毒汤对脂多糖诱导急性肺损伤小鼠的作用机制研究  

Mechanism of action of Qingfei Paidu decoction on mice with acute lung injury induced by lipopolysaccharide

作　　者：邱爱珠[1] 徐晔青 欧阳翌国[1] 张斌[1] 曾丹[1] 文芳 QIU Aizhu;XU Yeqing;OUYANG Yiguo;ZHANG Bin;ZENG Dan;WEN Fang(Hunan Traditional Chinese Medical College,Zhuzhou 412000,Hunan,China;Department of General Surgery,Zhuzhou Central Hospital,Zhuzhou 412000,Hunan,China)

机构地区：[1]湖南中医药高等专科学校,湖南株洲412000 [2]株洲市中心医院普外科,湖南株洲412000

出　　处：《湖南中医杂志》2022年第12期142-146,共5页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南中医药高等专科学校新冠肺炎科研专项(3)。

摘　　要：目的:探讨清肺排毒汤对脂多糖(LPS)诱导的急性肺损伤(ALI)小鼠的作用机制。方法:采用LPS经气道滴注建立小鼠ALI模型,造模成功后将小鼠随机分成治疗组和模型组,每组各10只,另取10只为空白对照组。治疗组给予清肺排毒汤0.3 ml灌胃,空白对照组、模型组给予0.9%氯化钠注射液0.3 ml灌胃,每隔8 h进行1次,共连续给药3次。采用HE染色观察肺组织病理变化情况并检测肺湿/干质量(W/D)比值,采用ELISA检测白细胞介素-1β(IL-1β)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)的含量。结果:与空白对照组比较,模型组肺组织结构破坏严重,肺泡间隔变宽,肺泡萎缩,模型构建成功;与模型组比较,治疗组肺组织结构破坏程度减轻,肺泡间隔厚度变小,充血水肿有所缓解。与模型组比较,治疗组肺组织W/D降低,1L-1β、TNF-α水平下降,1L-10水平上升,差异均有统计学意义(P<0.05)。结论:清肺排毒汤可能通过调节相关炎性因子表达,减轻炎性反应及肺水肿,对LPS诱导的急性肺损伤起到保护作用。Objective: To investigate the mechanism of action of Qingfei Paidu decoction on mice with acute lung injury(ALI) induced by lipopolysaccharide(LPS).Methods: A mouse model of ALI was established by airway dripping of LPS,and after successful modeling, the mice were randomly divided into treatment group and model group, with 10 mice in each group.Another 10 mice were selected as blank control group.The mice in the treatment group were given Qingfei Paidu decoction 0.3 ml by gavage, and those in the blank control group and the model group were given 0.9% sodium chloride injection by gavage, once every 8 hours for 3 consecutive times.HE staining was used to observe lung histopathological changes, and wet-to-dry lung weight ratio(W/D ratio) was measured;ELISA was used to measure the content of interleukin-1β(IL-1β),interleukin-10(IL-10),and tumor necrosis factor-α(TNF-α).Results: Compared with the blank control group, the model group had severe lung structural damage, widened alveolar septa, and atrophy of pulmonary alveoli, suggesting that the model was established successfully, and compared with the model group, the treatment group showed a lower degree of lung structural damage, a reduction in alveolar septal thickness, and alleviation of congestion and edema.Compared with the model group, the treatment group had significant reductions in W/D ratio, IL-1β,and TNF-α and a significant increase in IL-10(P<0.05).Conclusion: Qingfei Paidu decoction exerts a protective effect against LPS-induced ALI by regulating the expression of related inflammatory factors and alleviating inflammatory response and pulmonary edema.

关 键 词：急性肺损伤 清肺排毒汤 小鼠 炎症因子 作用机制 

分 类 号：R285.5[医药卫生—中药学]