脑胶质瘤组织中ZnT1 mRNA、miR-542-3p表达水平与病理特征的关系及对术后复发的预测价值  

Relationship between the expression levels of ZnT1 mRNA and miR-542-3p in glioma tissues and pathological characteristics and their predictive value for postoperative recurrence

作　　者：周龙 刘美霞 田仁富 潘轲 向春晖 ZHOU Long;LIU Meixia;TIAN Renfu;PAN Ke;XIANG Chunhui(Department of Neurosurgery,Enshi Tujia and Miao Autonomous Prefecture Central Hospital,Enshi,Hubei 445000,China;Department of Neurology,Enshi Tujia and Miao Autonomous Prefecture Central Hospital,Enshi,Hubei 445000,China)

机构地区：[1]恩施土家族苗族自治州中心医院神经外科,湖北恩施445000 [2]恩施土家族苗族自治州中心医院神经内科,湖北恩施445000

出　　处：《国际检验医学杂志》2023年第3期280-284,289,共6页International Journal of Laboratory Medicine

基　　金：湖北省卫生健康委员会医学科学研究课题计划项目(WJ2019M102)。

摘　　要：目的探讨脑胶质瘤组织中锌转运体1(ZnT1)信使RNA(mRNA)、微小RNA(miRNA)miR-542-3p表达水平与肿瘤病理特征的关系及对脑胶质瘤患者术后复发的预测价值。方法将2018年6月至2021年1月于该院行手术治疗的92例脑胶质瘤患者纳入研究。比较患者胶质瘤组织及瘤旁组织ZnT1 mRNA、miR-542-3p的表达水平。比较不同病理特征的瘤组织ZnT1 mRNA、miR-542-3p的表达水平。分析患者术后复发的影响因素。评估ZnT1 mRNA、miR-542-3p表达水平用于预测术后复发的价值。结果胶质瘤组织ZnT1 mRNA表达水平高于瘤旁组织(P<0.05),miR-542-3p表达水平低于瘤旁组织(P<0.05)。胶质瘤组织ZnT1 mRNA、miR-542-3p表达水平与病理分级、组织学分化、肿瘤浸润有关(P<0.05)。胶质瘤组织中ZnT1 mRNA高表达及miR-542-3p低表达会增加脑胶质瘤患者术后复发风险(P<0.05)。构建ZnT1 mRNA、miR-542-3p联合检测用于术后复发预测的方程,绘制预测术后复发的受试者工作特征(ROC)曲线,结果显示,这两项指标联合预测的效能高于单一指标预测(P<0.05)。结论脑胶质瘤组织中ZnT1 mRNA、miR-542-3p表达水平与病理分级、组织学分化、肿瘤浸润等病理特征有关,二者联合检测可用于预测患者术后复发。Objective To investigate the relationship between the expression levels of zinc transporter 1(ZnT1)messenger RNA(mRNA)and microRNA(miRNA)miR-542-3p in brain glioma tissues and the pathological characteristics of the tumors,and their predictive value for the recurrence of brain glioma patients after surgery.Methods A total of 92 patients with brain glioma who had undergone surgical treatment in the hospital from June 2018 to January 2021 were enrolled in the study.The expression levels of ZnT1 mRNA and miR-542-3p in glioma tissues and adjacent tissues of the patients were compared.The expression levels of ZnT1 mRNA and miR-542-3p in tumor tissues with different pathological characteristics were compared.The influencing factors of postoperative recurrence were analyzed.The value of the expression levels of ZnT1 mRNA and miR-542-3p in predicting postoperative recurrence was evaluated.Results The expression level of ZnT1 mRNA in glioma tissues was higher than that in adjacent tissues(P<0.05),and the expression level of miR-542-3p in glioma tissues was lower than that in adjacent tissues(P<0.05).The expression levels of ZnT1 mRNA and miR-542-3p in glioma were related to pathological grade,histological differentiation and tumor invasion(P<0.05).High expression level of ZnT1 mRNA and low expression level of miR-542-3p in glioma tissue increased the risk of recurrence of glioma patients after surgery(P<0.05).The equation for predicting postoperative recurrence by combined detection of ZnT1 mRNA and miR-542-3p was constructed,and the receiver operating characteristic(ROC)curve for predicting postoperative recurrence was drawn.The results showed that the prediction efficiency of the combined detection of two indicators was higher than that of single indicator(P<0.05).Conclusion The expression levels of ZnT1 mRNA and miR-542-3p in glioma tissue are related to pathological grading,histological differentiation,tumor invasion and other pathological characteristics.The combined detection of the two could be used to predict post

关 键 词：脑胶质瘤 锌转运体1 信使RNA miR-542-3p 病理特征 术后复发 

分 类 号：R446.8[医药卫生—诊断学]