不同施肥处理对杨树林地土壤微生物群落的影响  

Effects of Different Fertilization Treatments on Soil Microbial Communities in Poplar Forest Land

作　　者：孙慧 吴中能[1] 苗婷婷[1] 刘俊龙[1] 苏晓华[2] 丁昌俊[2] 曹志华[1] 闫彩霞 王涛 SUN Hui;WU Zhongneng;MIAO Tingting;LIU Junlong;SU Xiaohua;DING Changjun;CAO Zhihua;YAN Caixia;WANG Tao(Anhui Academy of Forestry Sciences,Hefei 230088;Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091;Bozhou Institute of Forestry Sciences,Bozhou,Anhui 233600)

机构地区：[1]安徽省林业科学研究院,合肥230088 [2]中国林业科学研究院林业研究所,北京100091 [3]亳州市林业科学研究所,安徽亳州233600

出　　处：《中国农学通报》2023年第2期36-43,共8页Chinese Agricultural Science Bulletin

基　　金：科技部“十四五”国家重点研发计划课题“杨树大径级工业资源材精准高效培育技术研究”(2021YFD2201200)。

摘　　要：土壤微生物多样性是提高人工林土壤生产力的重要因素之一,由于杨树二代林土壤退化严重,为了提高退化土地的土壤微生物多样性,找出合适的肥料配比方案,以安徽北方5年生杨树二代林为研究对象,进行连续4年的施肥试验后,采集土壤样品,采用高通量测序技术分析不同施肥处理杨树林地土壤微生物群落变化特征,并与环境因子进行RDA分析。结果表明:杨树二代林中,占优势细菌属的有MND1、Gaiella、小梨形菌属(Pirellula)及RB41,土壤表层施肥和未施肥土壤细菌菌属差异显著。蜡蚧菌属(Lecanicillium)、被孢霉属(Mortierella)及镰刀菌属(Fusarium)3种菌属为真菌优势菌属,真菌群落在土层中分布相关性较小。施用氮肥250 g/株、磷肥500 g/株、有机肥500 g/株及土壤改良生物菌肥200 g/株和氮肥125 g/株、磷肥500 g/株、钾肥75 g/株及土壤改良生物菌肥100 g/株其表层土壤的细菌分布较均匀且多样性较高,这两种施肥方案适合改善林地表层土壤细菌群落多样性。施用氮肥500 g/株、钾肥75 g/株、有机肥500 g/株及土壤改良生物菌肥150 g/株和氮肥250 g/株、磷肥750 g/株、钾肥25 g/株、土壤改良生物菌肥150 g/株其真菌在土壤深层的物种分布较均匀且多样性较高,这两种施肥方案适合改善林地深层土壤真菌群落多样性。脲酶对土壤细菌及真菌菌属群落组成影响均较大,是杨树林地重要的土壤环境因子。Soil microbial diversity is one of the important factors to improve soil productivity in planted forests.Due to the serious soil degradation of the second-generation Poplar forest and with the aim of improving the soil microbial diversity of degraded land and finding out the suitable ratio of fertilizer solution, 5-year-old second-generation Poplar forests in northern Anhui were used as the study objects. After 4 consecutive years of fertilization experiment, soil samples were collected, and high-throughput sequencing technology was used to analyze the change characteristics of soil microbial communities in Poplar forests under different fertilization treatments with an RDA analysis of environmental factors. The results showed that the MND1, Gaiella,Pirellula and RB41 were the dominant bacterial genera in the second-generation Poplar forest, and there were significant differences of bacterial genus between fertilized soil surface and unfertilized soil. Lecanicillium,Mortierella and Fusarium were 3 dominant fungal genera and the distribution of fungal communities in the soil layer was less relevant. Application of nitrogen fertilizer 250 g/plant, phosphate fertilizer 500 g/plant, organic fertilizer 500 g/plant and soil improvement biological bacterial fertilizer 200 g/plant and application of nitrogen fertilizer 125 g/plant, phosphate fertilizer 500 g/plant, potash fertilizer 75 g/plant and soil improvement biological bacterial fertilizer 100 g/plant had a more uniform distribution and higher diversity of bacteria in the surface soil, and were suitable for improving the diversity of forest surface soil bacterial community.Application of nitrogen fertilizer 500 g/plant, potash fertilizer 75 g/plant, organic fertilizer 500 g/plant and soil improvement biological bacterial fertilizer 150 g/plant and application of nitrogen fertilizer 250 g/plant,phosphate fertilizer 750 g/plant, potash fertilizer 25 g/plant and soil improvement biological bacterial fertilizer150 g/plant had a more uniform distribution of species and

关 键 词：杨树 肥料 微生物群落结构 土壤环境因子 RDA分析 

分 类 号：S792[农业科学—林木遗传育种]