平铺白珠种子萌发特性研究  

Study on Germination Characteristics of Gaultheria procumbens Seeds

作　　者：王思之 关文灵[1] 郝晓函 宋杰 WANG Sizhi;GUAN Wenling;HAO Xiaohan;SONG Jie(Flowers Research Institute,Yunnan Agricultural University,Kunming 650201;Flowers Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205)

机构地区：[1]云南农业大学花卉研究所,昆明650201 [2]云南省农业科学研究院花卉研究所,昆明650205

出　　处：《中国农学通报》2023年第1期77-84,共8页Chinese Agricultural Science Bulletin

基　　金：云南省现代农业花卉苗木产业体系建设“特色盆花良种快繁研究”(2017KJTX0010)。

摘　　要：为提高平铺白珠的繁殖效率,以采集的平铺白珠‘极速贵族’种子为供试材料,测量种子形态、千粒重、吸水特性;并通过培养皿滤纸萌发法统计不同GA3处理和温度处理下种子的发芽率;将无菌萌发所获幼苗与常规播种苗长势进行对比。结果表明,平铺白珠‘极速贵族’种子属小粒种子(千粒重(0.2995±0.0111)g),种子种皮薄且透水性良好。GA3浸种时间、GA3浸种浓度和温度三者间存在交互作用,其中GA3浓度和温度是影响种子萌发的关键因子,浓度为1000 mg/L的GA3溶液浸泡种子12~24 h,环境温度为白天25℃、夜晚15℃的条件有利于促进种子萌发,种子发芽率可达90%以上,显著高于对照(64.67%)。无菌苗长势要优于常规播种苗,无菌萌发1500 mg/L GA3预处理种苗的平均株高(12.17 mm)和平均根长(18.15 mm)均高于常规播种苗的株高(9.65 mm)和根长(13.31 mm),培养基配方为WPM+2.0 mg/L 6-BA+0.1 mg/L NAA+30 g/L蔗糖。To improve the reproduction efficiency of Gaultheria procumbens,the collected seeds of cultivar‘Speedy Baron’were used as test materials to measure seed morphology,thousand kernel weight,and water absorption characteristics.The germination rate of seeds under different GA3 treatments and temperature treatments were calculated by the Petri dish filter paper germination method.Finally,the growth of seedlings obtained from sterile germination was compared with that of conventional seedlings.The results showed that the seeds of G.procumbens‘Speedy Baron’were small seeds[thousand kernel weight(0.2995±0.0111)g],with thin seed coats and good water permeability.There were interactions among GA3 seed soaking time,GA3 seed soaking concentration and temperature,among which GA3 concentration and temperature were the key factors affecting seed germination.The seeds were soaked in GA3 solution with a concentration of 1000 mg/L for 12-24 h,and the ambient temperature was 25℃during the day and 15℃at night,which was conducive to promoting seed germination,and the seed germination rate could reach more than 90%,significantly higher than that of the control(64.67%).The growth of tissue cultured seedlings was better than that of conventional seedlings.The average plant height(12.17 mm)and average root length(18.15 mm)of sterile germination of 1500 mg/L GA3 pretreated seedlings were higher than those of conventional seedlings(plant height of 9.65 mm and average root length of 13.31 mm).The medium formula was WPM+2.0 mg/L 6-BA+0.1 mg/L NAA+30 g/L sucrose.

关 键 词：平铺白珠 种子萌发 种子特性 GA3 无菌萌发 

分 类 号：S604.1[农业科学—园艺学]