Near-infrared spectroscopy method for rapid proximate quantitative analysis of nutrient composition in Pacific oyster Crassostrea gigas  

作　　者：Zhe LI Haigang QI Ying YU Cong LIU Rihao CONG Li LI Guofan ZHANG 

机构地区：[1]CAS and Shandong Province Key Laboratory of Experimental Marine Biology,Center for Ocean Mega-Science,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [2]Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China [3]Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China [4]Public Technical Service Center,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [5]National&Local Joint Engineering Key Laboratory of Ecological Mariculture,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [6]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Oceanology and Limnology》2023年第1期342-351,共10页海洋湖沼学报（英文）

基　　金：Supported by the Shandong Province Key R&D Program Project(No.2021LZGC029);the Major Scientific and Technological Innovation Project of Shandong Province(No.2019JZZY010813);the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA24030105);the Qingdao Key Technology and Industrialization Demonstration Project(No.22-3-3-hygg-2-hy);the Earmarked Fund for China Agriculture Research System(No.CARS-49)。

摘　　要：Glycogen,amino acids,fatty acids,and other nutrient components affect the flavor and nutritional quality of oysters.Methods based on near-infrared reflectance spectroscopy(NIRS)were developed to rapidly and proximately determine the nutrient content of the Pacific oyster Crassostreagigas.Samples of C.gigas from 19 costal sites were freeze-dried,ground,and scanned for spectral data collection using a Fourier transform NIR spectrometer(Thermo Fisher Scientific).NIRS models of glycogen and other nutrients were established using partial least squares,multiplication scattering correction first-order derivation,and Norris smoothing.The R_(C) values of the glycogen,fatty acids,amino acids,and taurine NIRS models were 0.9678,0.9312,0.9132,and 0.8928,respectively,and the residual prediction deviation(RPD)values of these components were 3.15,2.16,3.11,and 1.59,respectively,indicating a high correlation between the predicted and observed values,and that the models can be used in practice.The models were used to evaluate the nutrient compositions of 1278 oyster samples.Glycogen content was found to be positively correlated with fatty acids and negatively correlated with amino acids.The glycogen,amino acid,and taurine levels of C.gigas cultured in the subtidal and intertidal zones were also significantly different.This study suggests that C.gigas NIRS models can be a cost-effective alternative to traditional methods for the rapid and proximate analysis of various slaughter traits and may also contribute to future genetic and breeding-related studies in Pacific oysters.

关 键 词：Pacific oyster Crassostrea gigas near-infrared reflectance spectroscopy(NIRS) nutrient composition rapid determination 

分 类 号：S917.4[农业科学—水产科学]