口罩中环氧乙烷残留量快速检测试剂盒的研制及应用  

Research and application of a rapid detection kit for the determination of ethylene oxide residue in masks

作　　者：李涛 王建山 刘海静 赵俊楠 LI Tao;WANG Jianshan;LIU Haijing;ZHAO Junnan(Shaanxi Institute for Food and Drug Control,Shaanxi Key Laboratory of Food and Drug Safety Monitoring,Xi'an 710065,China)

机构地区：[1]陕西省食品药品检验研究院,陕西省食品药品安全监测重点实验室,西安710065

出　　处：《分析试验室》2022年第11期1312-1316,共5页Chinese Journal of Analysis Laboratory

基　　金：2019年陕西省重点产业链(群)项目(2019ZDLSF07-08);2020年度陕西省药品监督管理局科学监管与监管科学研究项目资助。

摘　　要：样品中的环氧乙烷经盐酸溶液提取并水解成乙二醇,乙二醇经高碘酸氧化成甲醛,用硫代硫酸钠滴定除去过量的高碘酸,甲醛在碱性条件下与4-氨基-3-联氨-5-巯基-1,2,4-三氮杂茂(AHMT)发生缩合,再被高碘酸钾氧化成6-巯基-S-三氮杂茂[4,3-b]-S-四氮杂苯的紫红色络合物,其颜色的深浅在一定范围内与甲醛含量成线性关系,通过色阶溶液进行目视比色,对试样中环氧乙烷进行半定量测定。结果表明所研发的快检试剂盒检验结果与国标方法一致,灵敏度和特异性为100%,假阴性率和假阳性率为0。研制的快检试剂盒可实现口罩中环氧乙烷残留量的快速检测。Ethylene oxide in samples was extracted by the hydrochloric acid solution and converted to ethanediol.Ethanediol was then oxidized to oxymethylene by periodic acid and excess periodic acid was titrated by sodium thiosulfate.Oxymethylene reacted with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole(AHMT)under alkaline condition and then a kind of purple complex was formed after the products being oxidized by potassium periodate.The shade depth of the complex was in proportion to the concentration of oxymethylene.Then,the concentration of the ethylene oxide residue was determined by visual colorimetry.The results were consistent with that of national standard method.The sensitivity and specificity of the rapid detection kit were100%.The false negative and false positive rates were 0.The rapid detection kit developed was fast and convenient for the determination of the ethylene oxide residue in masks.

关 键 词：环氧乙烷 试剂盒 口罩 

分 类 号：R917[医药卫生—药物分析学]