CircRNA-0028171调控三氧化二砷诱导的血管内皮细胞凋亡  

CircRNA-0028171 regulates arsenic trioxide-induced apoptosis in vascular endothelial cells

作　　者：吴继臣 金赛迪 宋佳航 刘鑫琪 马文俊 常琳 官晓翔 张明宇[2] 刘嘉祺[1] 付惠 王莹[3] 许超千[2] WU Ji-Chen;JIN Sai-Di;SONG Jia-Hang;LIU Xin-Qi;MA Wen-Jun;CHANGLin;GUAN Xiao-Xiang;ZHANG Ming-Yu;LIU Jia-Qi;FU Hui;WANG Ying;XU Chao-Qian(College of Pharmacy,Mudanjiang Medical University,Mudanjiang 157400,China;College of Pharmacy,Harbin Medical University,Harbin 150081,China;College of Basic Medicine,Mudanjiang Medical University,Mudanjiang 157400,China)

机构地区：[1]牡丹江医学院药学院,牡丹江157400 [2]哈尔滨医科大学药学院,哈尔滨150081 [3]牡丹江医学院基础医学院,牡丹江157400

出　　处：《生理学报》2022年第5期763-772,共10页Acta Physiologica Sinica

基　　金：supported by the National Natural Science Foundation of China(No.82070240)。

摘　　要：本文旨在研究circ RNA-0028171对三氧化二砷(As_(2)O_(3))诱导的血管内皮细胞凋亡的调控作用。用0~15μmol/L As_(2)O_(3)作用人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVECs)24 h,用MTT法检测HUVECs损伤情况,用实时定量PCR检测circ RNA-0028171及凋亡相关蛋白Bcl-2、Bax m RNA表达水平,用Western blot检测Bcl-2、Bax蛋白表达水平。通过转染circ RNA-0028171过表达质粒和si RNA观察circ RNA-0028171是否参与As_(2)O_(3)对HUVECs的调控。结果显示,与对照组相比,As_(2)O_(3)组细胞活性显著下降,Bcl-2/Bax m RNA比值和蛋白比值显著降低,circ RNA-0028171显著低表达。过表达circ RNA-0028171抑制As_(2)O_(3)诱导的HUVECs凋亡,而敲减circ RNA-0028171促进As_(2)O_(3)诱导的HUVECs凋亡。上述结果提示,circ RNA-0028171参与调控As_(2)O_(3)诱导的血管内皮细胞凋亡过程。The present study was aimed to investigate the effects of circ RNA-0028171 on the apoptosis of vascular endothelial cells induced by arsenic trioxide(As_(2)O_(3)).Human umbilical vein endothelial cells(HUVECs)were treated with 0–15μmol/L As_(2)O_(3)for 24 h.Then,cellular viability was measured by MTT assay.The expression levels of circ RNA-0028171,Bcl-2 and Bax m RNA were detected by real-time quantitative PCR.Bcl-2/Bax protein ratio was detected by Western blot.Whether circ RNA-0028171 was involved in the regulation of HUVECs by As_(2)O_(3)was investigated by transfection with overexpression plasmid of circ RNA-0028171 and si RNA.The results showed that compared with the control group,As_(2)O_(3)group showed decreased cellular viability,reduced Bcl-2/Bax m RNA and protein ratios,and significantly lower expression of circ RNA-0028171.Overexpression of circ RNA-0028171 inhibited apoptosis of HUVECs induced by As_(2)O_(3).Knockdown of circ RNA-0028171 by si RNA promoted As_(2)O_(3)-induced apoptosis in HUVECs.These results suggest that circ RNA-0028171 is involved in the vascular endothelial cell apoptosis induced by As_(2)O_(3).

关 键 词：环状RNA 血管内皮细胞 三氧化二砷 凋亡 

分 类 号：R996[医药卫生—毒理学]