黄花蒿凝血药效部位及化学组分分析  

Analysis of the Coagulant Activity and Chemical Components in Artemisia annua L.

作　　者：赵发法 张晓蓉[2] ZHAO Fafa;ZHANG Xiaorong(College of Biology and Environmental Sciences,Jishou 416000,Hunan China;College of Pharmaceutical Sciences,Jishou University,Jishou 416000,Hunan China)

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]吉首大学药学院,湖南吉首416000

出　　处：《吉首大学学报（自然科学版）》2022年第5期30-36,共7页Journal of Jishou University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(51672104)。

摘　　要：对黄花蒿植物凝血物质基础进行了探究.采用有机溶剂提取法对黄花蒿植物进行浸提;采用凝血板法、试管法、血浆复钙时间测定法检测浸提物凝血药效;采用碱溶酸沉淀法及低温法对正丁醇浸提物的黄酮类化合物、萜类化合物进行分离并分析了黄酮类化合物紫外可见光谱及荧光光谱性质;采用理化显色反应对化合物进行鉴定,并利用TLC显色反应对萜类化合物进行分析.结果表明:黄花蒿植物凝血药效部位为正丁醇浸提物,正丁醇浸提物在不同的检测方法中均有显著的凝血药效,正丁醇浸提物主要化学组分为黄酮类化合物及萜类化合物.说明黄花蒿植物凝血药效部位为正丁醇浸提物,其化学组分主要为黄酮类化合物及萜类化合物.The pharmacodynamics substance in Artemisia annua L.was studied.The hemostasis chemical compositions were extracted by the organic solvent extraction method in Artemisia annua L..The hemostasis medicinal effect of the extract from Artemisia annua L.was detected using the coagulation plate method, the test tube method and the plasma recalcification time measurement method.The flavone compounds were separated from the butyl alcohol extract using Alkali dissolution-acid precipitation method.The spectroscopic properties were detected by the ultraviolet-visible spectroscopy and the fluorescence spectroscopy.And the flavone compounds were identified of the physical and chemical properties.The terpenoids compounds were separated from the butyl alcohol extract by the low temperature method, and were analyzed using the thin layer chromatography(TLC).The results indicated that the butyl alcohol extract was the hemostasis pharmacodynamics substance in Artemisia annua L..The clotting effect was significant of the butyl alcohol extract tested by the three methods of the coagulation plate method, the test tube method and the plasma recalcification time measurement method.And the chemical composition analysis showed that a lot of flavone compounds and the terpenoids compounds were contained in the butyl alcohol extract.It can be concluded that the pharmacodynamics substance is the butyl alcohol extract from Artemisia annua L.,and the major chemical constituents include the flavone compounds and the terpenoids compounds.

关 键 词：黄花蒿 药效物质基础 黄酮化合物 萜类化合物 

分 类 号：Q946.91[生物学—植物学]