CD4^(+)NKG2D^(+)T细胞在幼年特发性关节炎疾病活动中的作用  

Role of CD4^(+)NKG2D^(+)T cells in the disease activity of juvenile idiopathic arthritis

作　　者：王君言 朱晓萍 张宇[1] 罗冲[1] 唐雪梅[1] 周娟[1] WANG Jun-Yan;ZHU Xiao-Ping;ZHANG Yu;LUO Chong;TANG Xue-Mei;ZHOU Juan(Department of Rheumatology and Immunology,Children's Hospital of Chongqing Medical University/National Clinical Research Center for Child Health and Disorders/China International Science and Technology Cooperation Base of Child Development and Critical Disorders/Chongqing Key Laboratory of Child Infection and Immunity/Ministry of Education Key Laboratory of Child Development and Disorders,Chongqing 400014,China)

机构地区：[1]重庆医科大学附属儿童医院风湿免疫科/国家儿童健康与疾病临床医学研究中心/儿童发育重大疾病国家国际科技合作基地/儿童感染免疫重庆市重点实验室/儿童发育疾病研究教育部重点实验室,重庆400014 [2]贵州医科大学附属医院贵州省儿童医学中心,贵州贵阳550004

出　　处：《中国当代儿科杂志》2023年第2期166-171,共6页Chinese Journal of Contemporary Pediatrics

基　　金：国家重点研发计划项目(2021YFC2702003);重庆医科大学未来医学青年创新团队支持计划(W0114);重庆渝中区科技局科研项目(20210136)。

摘　　要：目的分析幼年特发性关节炎(juvenile idiopathic arthritis,JIA)活动期及稳定期CD4^(+)NKG2D^(+)T细胞及NKG2D可溶性配体,即可溶性MHC-Ⅰ类链相关分子A和B(soluble MHC classⅠchain-related molecules A/B,sMICA/sMICB)表达水平,探讨其在JIA疾病活动中的作用。方法前瞻性纳入2019年11月—2021年12月重庆医科大学附属儿童医院确诊的全身型JIA 19例和关节型JIA 20例,6例健康儿童为对照组。收集外周血标本,采用酶联免疫吸附法测定sMICA和sMICB水平,采用流式细胞术检测CD4^(+)NKG2D^(+)T细胞比例,采用全身型幼年关节炎疾病活动评分-27(systemic Juvenile Arthritis Disease Activity Score,sJADAS-27)/幼年关节炎疾病活动评分-27(Juvenile Arthritis Disease Activity Score-27,JADAS-27)评估JIA患儿的疾病活动性。采用Pearson相关分析法、受试者工作特征曲线分析CD4^(+)NKG2D^(+)T细胞及sMICA、sMICB在JIA疾病活动中的作用。结果全身型JIA活动期组及关节型JIA活动期组CD4^(+)NKG2D^(+)T细胞比例较对照组及各自稳定期组升高(P<0.05)。JIA各组sMICA和sMICB水平均高于对照组(P<0.05)。关节型JIA活动期组sMICB水平高于关节型JIA稳定期组(P<0.05)。JIA患儿CD4^(+)NKG2D^(+)T细胞比例及sMICA、sMICB水平与sJADAS-27/JADAS-27评分呈正相关(P<0.05)。sMICB对JIA疾病活动性评估的曲线下面积为0.755,特异度为0.90,灵敏度为0.64。结论JIA患儿CD4^(+)NKG2D^(+)T细胞比例及sMICA、sMICB水平较健康儿童升高,且与疾病活动性呈正相关,提示CD4^(+)NKG2D^(+)T细胞及NKG2D配体可作为JIA疾病活动性评估的新指标。Objective To study the expression levels of CD4^(+)NKG2D^(+)T cells and NKG2D soluble ligands,the soluble MHC classⅠchain-related molecules A and B(sMICA/sMICB)in the active stage and stable stage of juvenile idiopathic arthritis(JIA)and their role in the disease activity of JIA.Methods Nineteen children with systemic JIA and20 children with articular JIA who were diagnosed in Children’s Hospital of Chongqing Medical University from November 2019 to December 2021 were enrolled in this prospective study.Six healthy children were enrolled as the control group.After peripheral blood samples were collected,ELISA was used to measure the levels of sMICA and sMICB,and flow cytometry was used to measure the percentage of CD4^(+)NKG2D^(+)T cells.Systemic Juvenile Arthritis Disease Activity Score-27(sJADAS-27)/Juvenile Arthritis Disease Activity Score-27(JADAS-27)was used to evaluate the disease activity in children with JIA.The Pearson correlation analysis and the receiver operating characteristic(ROC)curve were used to assess the role of CD4^(+)NKG2D^(+)T cells,sMICA and sMICB in the disease activity of JIA.Results The active systemic JIA and active articular JIA groups had a significant increase in the percentage of CD4^(+)NKG2D^(+)T cells compared with the control group and their corresponding inactive JIA group(P<0.05).The JIA groups had significantly higher levels of sMICA and sMICB than the control group(P<0.05),and the active articular JIA group had a significantly higher level of sMICB than the stable articular JIA group(P<0.05).In the children with JIA,the percentage of CD4^(+)NKG2D^(+)T cells and the levels of sMICA and sMICB were positively correlated with sJADAS-27/JADAS-27 disease activity scores(P<0.05).The ROC curve analysis showed that sMICB had an area under the curve of0.755 in evaluating the disease activity of JIA,with a specificity of 0.90 and a sensitivity of 0.64.Conclusions The percentage of CD4^(+)NKG2D^(+)T cells and the levels of sMICA and sMICB increase in children with JIA compared with he

关 键 词：幼年特发性关节炎 自然杀伤细胞活化性受体2D 疾病活动性 儿童 

分 类 号：R725.9[医药卫生—儿科]