透明质酸功能修饰聚乳酸取向超细纤维促进血管平滑肌细胞的迁移  

Hyaluronic Acid-Modified Aligned Ultrafine Fibers of Poly(lactic acid)Promote Vascular Smooth Muscle Cell Migration

作　　者：岳蒙 张彦中[1] YUE Meng;ZHANG Yanzhong(College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学化学化工与生物工程学院,上海201620

出　　处：《功能高分子学报》2023年第1期77-85,共9页Journal of Functional Polymers

基　　金：国家自然科学基金(32071345、31771050);东华大学中央高校基本科研业务费学科交叉(理工科)重点计划项目(2232019A3-09);国家重点研发计划专项(2016YFC1100203)。

摘　　要：基于稳定射流同轴电纺丝法(SJCES),选用透明质酸(HA)对电纺左旋聚乳酸(PLLA)取向超细纤维进行表面功能修饰得到壳-芯结构的取向纤维(HA-PLLA),以提高血管平滑肌细胞(vSMCs)在纤维表面的黏附和迁移能力。首先在无血清饥饿处理条件下观察HA促进vSMCs迁移的功效;然后采用扫描电子显微镜(SEM)、透射电子显微镜(TEM)、红外光谱仪(FT-IR)、X-射线衍射仪(XRD)和万能材料测试仪等对纤维形貌、壳-芯结构和力学性能进行表征;最后通过改良版“伤口”愈合实验检测HA-PLLA取向纤维诱导vSMCs迁移的功效。结果表明:HA具有显著促进vSMCs迁移的作用;SJCES法可成功制备HA-PLLA壳-芯取向纤维;HA修饰可显著提高PLLA取向纤维的细胞相容性,并增强其定向引导vSMCs迁移的能力,从而证实了HAPLLA取向纤维促进vSMCs迁移的有效性。To improve the capacity of adhesion and migration in vascular smooth muscle cells(vSMCs),the fiber surface was modified by having the electrospun aligned poly(lactic-lactide)(PLLA)ultrafine fibers individually coated with hyaluronic acid(HA)biomacromolecules via stable jet coaxial electrospinning(SJCES).First of all,under the condition of serum-free starvation,the effect of HA presence in the culture medium on promoting vSMC migration was examined.Then,highly-aligned HA-coated PLLA composite fibers(HA-PLLA)in shell-core structure were produced by SJCES,and the morphology,shell-core structure and mechanical properties of the HA-PLLA fibers were characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM),infrared spectroscopy(FT-IR),X-ray diffraction(XRD),and a universal mechanical tester.Finally,the efficacy of HA-PLLA fiber arrays in supporting vSMC migration was detected based on an improved"wound"healing method.Results showed that the presence of HA was capable of promoting vSMC migration.HA-PLLA aligned fibers in shell-core structure were successfully fabricated using SJCES.Modification of HA significantly improved the cytocompatibility of the aligned PLLA fibers,and enhanced their efficiency in directing vSMC migration,thus confirming the effectiveness of HA modification in promoting the migration of vSMCs on the aligned fibrous substrate of PLLA.

关 键 词：血管组织工程 左旋聚乳酸 透明质酸 电纺取向纤维 平滑肌细胞 细胞迁移 

分 类 号：R318.08[医药卫生—生物医学工程]