G-三聚体:发现、表征和分析应用  

G-triplexes:discovery,characterization and analytical application

作　　者：赵玲丽 周颖琳[2] 张新祥[2] ZHAO Lingli;ZHOU Yinglin;ZHANG Xinxiang(Department of Chemistry,,Zhejiang University,Hangzhou 310012,China;Beijing National Laboratory for Molecular Sciences(BNLMS),MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China)

机构地区：[1]浙江大学化学系,杭州310012 [2]北京分子科学国家研究中心,生物有机与分子工程教育部重点实验室,北京大学化学与分子工程学院,北京100871

出　　处：《分析试验室》2022年第12期1379-1390,共12页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(22174002,22076003,21675004)项目资助。

摘　　要：G-三聚体是近年来被确认的一种新型核酸结构,由富含鸟嘌呤(G)的核酸单链通过Hoogsteen氢键稳定的G-三分体平面堆叠形成。在G-四聚体结构折叠途径的研究中,G-三聚体作为中间体被假设存在,随后通过计算机模拟技术被观察到,其存在性通过实验而得到验证。G-三聚体一经发现就引起了人们极大的兴趣。研究人员通过多种生物物理技术对G-三聚体结构和功能开展深入研究,不仅拓展了人们对于核酸生物学功能的理解和认识,还打开了G-三聚体在生化领域应用的新大门。本文主要介绍G-三聚体的发现过程,其结构与性质的表征,并综述其在生化分析中的应用研究进展。G-triplexes have been identified as a new category of nucleic acid structures.G-triplexes are guanine(G)-rich sequences that comprise the G-triad planes stabilized by Hoogsteen hydrogen bonds.For a long time,Gtriplex structure has been already hypothesized in the investigations on the folding pathway of G-quadruplexes.Then computational modeling and simulation methods were used to predict the potential formation of G-triplex structure.Eventually,this structure was experimentally proven.G-triplexes had received growing research attention since their discovery.Structural and functional characterization of these G-triplexes have been conducted through a combination of biophysical experiments.Accumulating knowledge about G-triplexes has helped to expand the understanding of the biological functions of nucleic acids and has opened new doors to the applications of G-triplexes in biological areas.In this paper,the discovery process of G-triplex,the characterization of its structure and properties,and its application in biochemical analysis were reviewed.

关 键 词：G-三聚体 G-四聚体 分子模拟 圆二色 核磁共振 生物传感器 

分 类 号：O657[理学—分析化学]