基于纳米探针形貌变化的碱性磷酸酶活性检测方法研究进展  

Research progress on alkaline phosphatase activity assays based on the morphological changes of nanoprobes

作　　者：黄敏 郑文飞 程茹 祝福 王伟 王健[1] HUANG Min;ZHENG Wenfei;CHENG Ru;ZHU Fu;WANG Wei;WANG Jian(Key Laboratory of Luminescence Analysis and Molecular Sensing,Ministry of Education,College of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China;Shandong Qingdao West Coast New Area People's Hospital,Qingdao 266499,China)

机构地区：[1]发光分析与分子传感教育部重点实验室,西南大学药学院,重庆400715 [2]山东省青岛西海岸新区人民医院,青岛266499

出　　处：《分析试验室》2022年第12期1391-1399,共9页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金面上项目(22174115);重庆市自然科学基金面上项目(cstc2019jcyjmsxmX0279);大学生创新创业训练计划项目(S202210635274)资助。

摘　　要：碱性磷酸酶(ALP)不仅是一种重要的生物标志物,还是分子生物学和酶免疫分析中的重要工具酶。因此,建立灵敏、准确的ALP活性检测方法对疾病的诊断及生物医学具有重要意义。迄今为止,研究人员已经发展了许多检测ALP活性的分析方法,包括比色法和散射法等,这些方法的分析性能依赖于探针的特性。Au,Ag,Cu等贵金属纳米材料具有很强的局域表面等离激元共振(LSPR)效应,其特征吸收及颜色随形貌的变化而变化,可用于发展比色法检测ALP的活性。此外,纳米探针的暗场光散射同样具有形貌依赖性,可根据纳米材料形貌引起的散射光谱及散射光颜色的变化,建立单颗粒分析法以实现ALP活性的灵敏检测。目前,改变纳米探针的形貌主要有刻蚀、生长等方式,均可用于发展分析ALP活性的方法。本文对基于纳米探针形貌变化的ALP活性检测方法进行总结,并探讨ALP活性检测的提升空间,希望能为今后的相关工作提供一些启发。Alkaline phosphatase(ALP)is not only an important biomarker,but also a vital tool enzyme in molecular biology and enzyme immunoassay.Therefore,the development of sensitive and accurate methods to detect ALP activity is of great significance for disease diagnosis and biomedicine.So far,a large number of analytical methods have been developed for ALP activity detection,including colorimetry,light scattering technology and so on.The analytical performance of these methods is highly dependent on the probe property.Gold,silver,copper and other noble metal nanomaterials present strong localized surface plasmon resonance(LSPR)properties,and the characteristic absorption and color are highly dependent on their morphology,which can be used to develop colorimetric methods for ALP activity detection.The dark-field light scattering of nanoprobe is also morphology-dependent.Based on the light scattering spectra and color changes caused by the morphological change of nanomaterials,the highly sensitive assays can be established to realize ALP activity detection at single-particle level.At present,a wide range of assays for ALP activity detection have been developed based on the nanoprobes morphology change,including etching or growth,and so on.This review summarized ALP activity detection methods based on the morphological changes of nanoprobes,and discussed the improvement of ALP activity detection.It is hoped to provide some inspiration for related work in the future.

关 键 词：碱性磷酸酶 光谱法 纳米探针 形貌变化 暗场显微成像技术 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]