两株GI.1型札如病毒全基因组测序及初步分析  

Whole genome sequencing and preliminary analysis of two strains of GI.1 sapovirus

作　　者：蒋翠莲 曹雪[2] 艾静[3] 付建光[3] Jiang Cuilian;Cao Xue;Ai Jing;Fu Jianguang(Clinical Laboratory,Nanjing Maternity and Child Health Care Hospital,Nanjing 210004,China;Department of Immunology,Nanjing Medical University,211166,China;Institute of Acute Infectious Disease Prevention and Control,Jiangsu Provincial Center for Disease Control and Prevention,210009,China)

机构地区：[1]南京医科大学附属妇产医院、南京市妇幼保健院检验科,210004 [2]南京医科大学免疫学教研室,211166 [3]江苏省疾病预防控制中心急性传染病防制所,210009

出　　处：《国际病毒学杂志》2022年第5期408-411,共4页International Journal of Virology

基　　金：南京市医学科技发展项目(YKK17183)。

摘　　要：目的对GI.1型札如病毒进行全基因组扩增测序尝试并开展初步分析。方法利用本研究设计的GI组札如病毒全基因组5’末端通用引物及杯状病毒3’末端引物,对本实验室保存的两株GI.1型札如病毒标本进行全基因组扩增,然后进行文库构建、上机测序及序列组装。通过BioEdit软件进行序列比对分析,采用MEGA 7.0软件构建进化树进行进化分析。结果两个标本测序质量优异,测序方法具有可行性;在全基因组(WGS)、NSP基因、VP1基因及VP2基因进化树中,均归类为GI.1型;位点变异分布于各个基因片段,但VP1基因的N端保守性较好,熵值较低;大部分基因的核苷酸突变尤其是NS3、NS5及VP1基因的核苷酸突变以同义突变为主,未形成相应氨基酸的突变。结论成功地开展了对GI.1型札如病毒全基因组扩增测序及相关进化分析,为札如病毒的检测提供了新的思路。Objective To explore the feasibility of whole genome amplification and sequencing of GI.1 sapovirus and carry out preliminary analysis.Methods The universal primers at 5'end of sapovirus genogroup I and the primers at 3’end of Calicivirus were designed and the whole genomes of two strains of GI.1 sapovirus stored in our laboratory were amplified followed by library construction,sequencing and sequences assembly.Sequence alignment analysis was carried out by BioEdit software,and phylogenetic tree was constructed by MEGA 7.0 software for evolutionary analysis.Results The sequencing quality of the two samples was excellent,and the sequencing method was feasible.According to phylogenetic trees of whole genome,NSP gene,VP1 gene and VP2 gene,both strains were classified as GI.1 genotype.Point mutations were observed in every gene segment,but the N-terminal of VP1 gene was well conserved and the entropy value was low.Most nucleotide mutations,especially those in NS3,NS5 and VP1 genes were mainly synonymous mutations and no corresponding amino acid mutations were found.Conclusions The whole genome amplification,sequencing and phylogenetic analysis of GI.1 sapovirus were achieved.This provides a new route for sapovirus detection.

关 键 词：札如病毒 全基因组 进化树 同义突变 

分 类 号：R373[医药卫生—病原生物学]